Project description:Transcriptome sequencing of Foxtail millet Setaria italica (Zhang-gu) for different tissues. Four RNA pools were created corresponding to four different tissues: root, leaf, stem, spica (tassel) at developmental stage, then each pool was sequenced.
Project description:Foxtail millet (Setaria italica L. P. Beauv) has been considered as a tractable model crop in recent years due to its short growing cycle, lower repetitive DNA, inbreeding nature, small diploid genome, and outstanding abiotic stress-tolerance characteristics. With modern agriculture often facing various adversities, it’s urgent to dissect the mechanisms of how foxtail millet responds and adapts to drought and stress on the proteomic-level.
Project description:Shotgun analysis was employed to obtain the proteome of two foxtail millet (Setaria italica) varieties, Ci846 and Yugu18, at the jointing stage. Whole proteins were extracted from internodes, nodes, and leaves, respectively. Eluted peptides were analyzed using a Q Exactive HF instrument (Thermo Fisher, USA).
