Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Next Generation Sequencing Facilitates Quantitative Analysis of Vehicle or R-2-HG Treating Mouse Peritoneal Macrophages Transcriptomes

ABSTRACT: Next Generation Sequencing Facilitates Quantitative Analysis of Vehicle or R-2-HG Treating Mouse Peritoneal Macrophages Transcriptomes

PROVIDER: PRJNA751599 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR15327281_1.fastq.gz	Fastqsanger.gz
	SRR15327281_2.fastq.gz	Fastqsanger.gz
	SRR15327282_1.fastq.gz	Fastqsanger.gz
	SRR15327282_2.fastq.gz	Fastqsanger.gz
	SRR15327283_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 12

Similar Datasets

Next Generation Sequencing Facilitates Quantitative Analysis of Vehicle or R-2-HG Treating Mouse Peritoneal Macrophages Transcriptomes

Project description:Purpose: The goals of this study are to screen differentially expressed genes in mouse peritoneal macrophages treated by vehicle or R-2-HG and to explore potential mechanisms of how R-2-HG regulating the process of macrophage activation. Methods: After RNA extraction, purification, and library construction, the samples were paired-end (PE) sequenced by Next-Generation Sequencing using an Illumina sequencing platform (Illumina HiSeq 2500). After sequencing, the filtered high-quality sequences (Clean Data) filtered from the original raw data were mapped to the Mus_musculus GRCm38.p4 (mm10) genome. Based on the mapped results, the expression level of each gene was calculated, and the samples of the different groups were subjected to further analysis for differences in expression, enrichment, and cluster analysis. Results: The mRNA expression levels of only a few genes were changed after R-2-HG treatment in mouse peritoneal macrophages, but HIF1A was not included. Conclusions:Regulating the macrophage activation by R-2-HG may not be due to its effect on gene transcription, and R-2-HG doses not affect the mRNA expression of HIF1A.

2024-07-31 | GSE181340 | GEO

MOUSE peritoneal macrophages treated with LUP or Vehicle

Project description:Mouse peritoneal macrophages were isolated and treated with vehicle or LUP (0.5 mg/ml) for 24 h.

2024-03-17 | GSE237853 | GEO

MOUSE peritoneal macrophages treated with LUP or Vehicle

Project description:MOUSE peritoneal macrophages treated with LUP or Vehicle

| PRJNA996941 | ENA

Transcriptomic profile of trametinib on LPS-mediated gene expression in murine peritoneal macrophages

Project description:To determine the underlying mechanisms by which trametinib affected LPS-induced inflammatory response in macrophages, we performed microarrays to define the global gene expression in murine peritoneal macrophages treating with trametinib or vehicle followed by LPS stimulation.

2021-07-12 | GSE134486 | GEO

Next Generation Sequencing Facilitates Quantitative Analysis of Peritoneal Macrophage Transcriptomes

Project description:Next Generation Sequencing Facilitates Quantitative Analysis of Peritoneal Macrophage Transcriptomes

| PRJNA512475 | ENA

MOUSE peritoneal macrophages treated with C498 or LPS

Project description:Mouse peritoneal macrophages were isolated and treated with vehicle or C498 (15 μM) for 0.5 h, followed by LPS (100 ng/ml) challenge for an additional 4 h.

2023-05-03 | GSE220654 | GEO

Comparative gene expression profiling analysis of RNA-seq data for Primary peritoneal macrophages

Project description:Primary peritoneal macrophages were obtained from the peritoneal exudates of mice that were pretreated with 3% thioglycolate (LA4590, Solarbio) i.p. for 5 days. The peritoneal exudate cells were collected by cold PBS and adjusted to 1 × 10^6 cells/ml in DMEM cultured at 37C and 5% CO2 in a cell incubator. At 2 hours later, after using PBS to wash out the supernatant cells, the remaining adherent cells contained more than 90% F4+/80+ macrophages by flow cytometry analysis. Peritoneal macrophages were treated with 100ng/ml of recombinant murine BMP9 or Vehicle for 6h to activate macrophages.Then, send to RNA seq,

2024-02-18 | GSE230030 | GEO

Heliocidaris sp. HG-2022

Project description:Heliocidaris sp. HG-2022 Genome sequencing

| PRJNA869508 | ENA

RNA-sequence facilitates analysis of mesenchymal stem cells treating with concanavalin A mice

Project description:RNA-sequence facilitates analysis of mesenchymal stem cells treating with concanavalin A mice

| PRJNA740028 | ENA

Streptomyces sp. HG-16

Project description:Streptomyces sp. HG-16 Genome sequencing and assembly

| PRJNA511791 | ENA