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MOUSE peritoneal macrophages treated with LUP or Vehicle

ABSTRACT: MOUSE peritoneal macrophages treated with LUP or Vehicle

PROVIDER: PRJNA996941 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR25373422_1.fastq.gz	Fastqsanger.gz
	SRR25373422_2.fastq.gz	Fastqsanger.gz
	SRR25373423_1.fastq.gz	Fastqsanger.gz
	SRR25373423_2.fastq.gz	Fastqsanger.gz
	SRR25373424_1.fastq.gz	Fastqsanger.gz

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MOUSE peritoneal macrophages treated with LUP or Vehicle

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Project description:We have identified a number of miRNAs that are differentially expressed in LPS treated mouse peritoneal macrophages, as compared to untreated cells Peritoneal macrophages treated with LPS for 0, 4, 12, 24h. RNA was isolated and miRNA array assays were performed by Exiqon (miRCURY™ LNA Array version 10.0)

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Next Generation Sequencing Facilitates Quantitative Analysis of Vehicle or R-2-HG Treating Mouse Peritoneal Macrophages Transcriptomes

Project description:Purpose: The goals of this study are to screen differentially expressed genes in mouse peritoneal macrophages treated by vehicle or R-2-HG and to explore potential mechanisms of how R-2-HG regulating the process of macrophage activation. Methods: After RNA extraction, purification, and library construction, the samples were paired-end (PE) sequenced by Next-Generation Sequencing using an Illumina sequencing platform (Illumina HiSeq 2500). After sequencing, the filtered high-quality sequences (Clean Data) filtered from the original raw data were mapped to the Mus_musculus GRCm38.p4 (mm10) genome. Based on the mapped results, the expression level of each gene was calculated, and the samples of the different groups were subjected to further analysis for differences in expression, enrichment, and cluster analysis. Results: The mRNA expression levels of only a few genes were changed after R-2-HG treatment in mouse peritoneal macrophages, but HIF1A was not included. Conclusions:Regulating the macrophage activation by R-2-HG may not be due to its effect on gene transcription, and R-2-HG doses not affect the mRNA expression of HIF1A.

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Gene expression profile (RNAseq) of wild-type and Zeb1 (+/-) peritoneal mouse macrophages

Project description:Purpose: Characterize the gene expression profile of wild-type and Zeb1 (+/-) peritoneal mouse macrophages through RNA sequencing Methods: RNA sequencing of RNA from peritoneal macrophages isolated by peritoneal lavage and FACS sorting (F4/80+ CD11b+) from wild-type and Zeb1 (+/-) mice (2 mice pooled for each sample/set) Results: Wild-type and Zeb1 (+/-) peritoneal mouse macrophages display differential gene expression. Zeb1 (+/-) peritoneal macrophages express lower levels of SPM (F4/80low)-associated genes Conclusions: Wild-type and Zeb1 (+/-) peritoneal mouse macrophages display differential gene expression. Zeb1 (+/-) peritoneal macrophages express lower levels of SPM (F4/80low)-associated genes

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Gene expression profile (RNAseq) of peritoneal mouse macrophages in Endotoxic shock and Tolerance

Project description:Purpose: Characterize the gene expression profile of of peritoneal mouse macrophages in Endotoxic shock and Tolerance through RNA sequencing Methods: RNA sequencing of RNA from peritoneal macrophages in Endotoxic shock and Tolerance isolated by peritoneal lavage and FACS sorting (F4/80+ CD11b+) Results: Endotoxic shock and Tolerance peritoneal mouse macrophages display differential gene expression. Conclusions: Endotoxic shock and Tolerance peritoneal mouse macrophages display differential gene expression.

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Project description:Primary peritoneal macrophages were obtained from the peritoneal exudates of mice that were pretreated with 3% thioglycolate (LA4590, Solarbio) i.p. for 5 days. The peritoneal exudate cells were collected by cold PBS and adjusted to 1 × 10^6 cells/ml in DMEM cultured at 37C and 5% CO2 in a cell incubator. At 2 hours later, after using PBS to wash out the supernatant cells, the remaining adherent cells contained more than 90% F4+/80+ macrophages by flow cytometry analysis. Peritoneal macrophages were treated with 100ng/ml of recombinant murine BMP9 or Vehicle for 6h to activate macrophages.Then, send to RNA seq,

2024-02-18 | GSE230030 | GEO

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