Project description:Medial ganglionic eminence was dissected from e14.5 mouse embryonic brain, dissociated and processed using 10X Genomics chemistry for single cell library creation. 2 experimental groups were analyzed: St18 null and wildtype littermate controls (2 biological replicates of each group)
Project description:This study aimed to understand the role of the transcriptional regulator Prdm16 in the development of cortical interneurons in the mouse. Prdm16 was knocked out in cells derived from the medial ganglionic eminence (MGE) by using an Nkx2.1-Cre driver line in combination with a line carrying floxed Prdm16 alleles and with a Cre-dependent tdTomato reporter line (Ai14). The sequencing data compares the gene expression profiles of dissected MGEs at embryonic day 14 (E14), a stage when cortical interneurons are being generated from MGE progenitors.
Project description:We studied the membrane protein compositions of Streptococcus pneumoniae WT and scRNA mutant strains, with or without the CSP1 induction into competence state.
Project description:In oroder to understand the maturation process of pre-HSCs to HSC, we conducted scRNA-seq using E11.5pre-HSCs and E12.5&E14.5 LT_HSC populations. Pseudotime analysis and velocity analysis show the progression of pre-HSC to mature HSCs and also divergent point of lineage trajectory.