Ontology highlight
ABSTRACT:
PROVIDER: PRJNA751694 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR15334816_1.fastq.gz | Fastqsanger.gz | |||
| SRR15334816_2.fastq.gz | Fastqsanger.gz |
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Similar Datasets
Project description:Background: Treponema denticola is strongly associated with the development of periodontal disease. Both synergistic and antagonistic effects are observed among bacterial species in the process of biofilm formation. Bacteriocin-related genes have not yet been fully characterized in periodontopathic bacteria. The aim of this study was to detect and characterize bacteriocin-associated proteins in T. denticola. Methods: The whole genome sequence of T. denticola ATCC 35405 was screened with a Streptococcus mutans bacteriocin immunity protein (ImmA/Bip) sequence. The prevalence of homologous genes in T. denticola strains was then investigated by Southern blotting. Expression of the genes was evaluated by qRT-PCR. Results: In the genome sequence of T. denticola, an amino acid sequence coded by open reading frame TDE_0719 showed 26% identity with the S. mutans ImmA. Furthermore, two protein sequences coded by TDE_0425 and TDE_2431 in T. denticola ATCC 35405 showed ~40% identity with that coded by TDE0719. Therefore, TDE_0425, TDE_0719, and TDE_2431 were designated as tepA1, A2, and A3, respectively. Open reading frames showing similarity to the HlyD family of secretion proteins were detected downstream of tepA1, A2, and A3. They were designated as tepB1, B2, and B3, respectively. A gene harboring a bacteriocin-like signal sequence was detected upstream of tepA1. The prevalence of tepA1 and A2 differed among Treponema species. Susceptibility to chloramphenicol and ofloxiacin was slightly decreased in a tepA2 mutant while that to kanamycin was increased. Expression of tepA3-B3 was increased in the tepA2 mutant. Conclusion: These results indicate that T. denticola ATCC 35405 has three potential bacteriocin export proteins and that the presence of these genes differs among the Treponema strains. These proteins may be involved in resistance to chloramphenicol.
2017-01-01 | GSE83445 | GEO
Project description:Treponema denticola is involved in chronic periodontitis pathogenesis. The mechanism underlying the regulation of the expression of its virulence factors, such as major surface protein (Msp) is yet to be clarified. We determined the gene expression profiles of Msp-deficient mutants of T. denticola. Gene expression profiles of T. denticola ATCC 35405 (wild type), and msp-deficient mutant, DMSP3, were determined using DNA microarray analysis. In addition to several differentially expressed genes, dentilisin expression was reduced in DMSP3. Potential repressor, TDE_0344, was elevated in DMSP3.
2021-02-27 | GSE159241 | GEO
Project description:Porphyromonas gingivalis and Treponema denticola are periodontalpathogens that are associated with the severity and progression of periodontal diseases. This study investigates the gene expression of Porphyromonas gingivalis during co-culture with Treponema denticola
2014-03-31 | E-MTAB-2214 | biostudies-arrayexpress
Project description:Porphyromonas gingivalis and Treponema denticola are periodontalpathogens that are associated with the severity and progression of periodontal diseases. this study investigates the gene expression of Treponema denticola during co-culture with Porphyromonas gingivalis.
2014-03-31 | E-MTAB-2257 | biostudies-arrayexpress
Project description:In this project, we investigated whether diverse spirochete species contain lysinoalanine cross-linkages between conserved residues in their flagellar hook proteins (FlgE). We examined the following species: Treponema denticola¸ Borreliella burgdorferi, Treponema phagedenis, Treponema pallidum, Brachyspira hyodysenteriae, Leptospira interrogans. For each bacterial species, we examined a variety of different sample types, including recombinantly produced FlgE, polyhooks purified from flik knock-out strains, and wild-type periplasmic filaments. Overall, our data suggests that lysinoalanine crosslinking in a conserved FlgE post-translational modification in spirochetes and is required for the unique motility of these organisms. For more information, please see the following manuscript: https://doi.org/10.1101/2023.06.13.544825.
2024-01-26 | PXD045997 | Pride
Project description:The objectives of this investigation were to examine changes in the host transcriptional profiles during a polymicrobial periodontal pathogens Porphyromonas gingivalis, Treponema denticola, and Tannerella forsythia infection using a murine calvarial model of inflammation and bone resorption. P. gingivalis FDC 381, T. denticola ATCC 35404, and T. forsythia ATCC 43037 was injected into the subcutaneous soft tissue over the calvaria of BALB/c mice for 3 days, after which the soft tissues and calvarial bones were excised. RNA was isolated from infected soft tissues and calvarial bones and analyzed for transcript profiles using Murine GeneChip® MG-MOE430A Affymetrix arrays to provide a molecular profile of the events that occur following infection of these tissues. We used mouse microarrays to detail the molecular profile of the events that occur following infection of calvarial and bone tissues and identified distinct classes of up- and down-regulated genes during this process.
2014-01-10 | GSE29670 | GEO
Project description:Treponema denticola is a major etiological agent of chronic periodontis. Dentipain is one of the virulence factors of this microorganism. The localization and function of the protein has yet to be clarified although it has shown to be involved in the virulence of T. denticola. Therefore, this study aimed to characterize the role of dentipain in T. denticola. To investigate the function of dentipain, dentipain deficient mutant was constructed using an ermFermAM cassette and gene expression profile of the mutant was evaluated by microarray analysis. Growth rate of the dentipain-deficient mutants was smaller than that of the wild-type strain. In the mutant. Thirty-five genes in differentially expressed genes by the microarray analysis annotated as membrane or plasma membrane in gene ontology. Hydrophobicity of the mutant were higher than that of the wild-type strain. These findings suggest that dentipain involved in the surface characteristics and growth of T. denticola.
2023-05-17 | GSE213450 | GEO
Project description:The objectives of this investigation were to examine changes in the host transcriptional profiles during a Treponema denticola infection using a murine calvarial model of inflammation and bone resorption. T. denticola ATCC 35404 was injected into the subcutaneous soft tissue over the calvaria of BALB/c mice for 3 days, after which the soft tissues and calvarial bones were excised. RNA was isolated from infected soft tissues and calvarial bones and analyzed for transcript profiles using Murine GeneChip® MG-MOE430A Affymetrix arrays to provide a molecular profile of the events that occur following infection of these tissues. We used mouse microarrays to detail the molecular profile of the events that occur following infection of calvarial and bone tissues and identified distinct classes of up- and down-regulated genes during this process.
2010-11-16 | GSE19855 | GEO
Project description:Treponema denticola ATCC 35405 Transcriptome or Gene expression
| PRJNA270691 | ENA