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Toxicogenomic responses in sheepshead minnow (Cyprinodon variegatus) exposed to polyethylene terephthalate (PET) microfibers

ABSTRACT: Toxicogenomic responses in sheepshead minnow (Cyprinodon variegatus) exposed to polyethylene terephthalate (PET) microfibers

PROVIDER: PRJNA753063 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR15374306_1.fastq.gz	Fastqsanger.gz
	SRR15374306_2.fastq.gz	Fastqsanger.gz
	SRR15374307_1.fastq.gz	Fastqsanger.gz
	SRR15374307_2.fastq.gz	Fastqsanger.gz
	SRR15374308_1.fastq.gz	Fastqsanger.gz

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Toxicogenomic responses in sheepshead minnow (Cyprinodon variegatus) exposed to polyethylene terephthalate (PET) microfibers

Project description:Purpose: To analyze the toxicity effect of sheepshead minnow larvae exposed to PET microfibers through RNA seq. The expression of specific and sensitive genes on PET microfibers was confirmed and verified through qRT-PCR. Method: RNA was isolated from a sample exposed to PET microfibers (concentration of 10, 100 mg/L) for 10 days using a 72 hpf sheepshead minnow larvae. mRNA was produced with a TruSeq Standed mRNA library kit, and sequencing using a NovaSeq6000 equipment. qRT–PCR validation was performed using SYBR Green assays Results and Conclusions: Our study represents a detailed analysis of the transcriptome of sheepshead minnow larvae exposed to PET microfibers by RNA-seq technology. Through the 10 and 100 mg/L PET exposure, 670 differentially expressed genes confirmed. These genes that are specific and sensitive to PET microfibers are concentrated in Dendrite morphogenesis, heart development, intracellular protein kinase cascade, cell migration, and cardiac muscle cell development through GO analysis. In addition, the changes in cancer genesis, cell reaction, energy, and neuronal related genes were confirmed.

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