Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

High-throughput RNA sequencing analysis of mouse peritoneal cells from wild type and Lactoferrin knockout mouse during Lipopolysaccharide(LPS) stimulation

ABSTRACT: High-throughput RNA sequencing analysis of mouse peritoneal cells from wild type and Lactoferrin knockout mouse during Lipopolysaccharide(LPS) stimulation

PROVIDER: PRJNA753295 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR15402136_1.fastq.gz	Fastqsanger.gz
	SRR15402136_2.fastq.gz	Fastqsanger.gz
	SRR15402137_1.fastq.gz	Fastqsanger.gz
	SRR15402137_2.fastq.gz	Fastqsanger.gz
	SRR15402138_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 24

Similar Datasets

High-throughput RNA sequencing analysis of mouse peritoneal cells from wild type and Lactoferrin knockout mouse during Lipopolysaccharide(LPS) stimulation

Project description:Purpose: The goal of this study is using RNA sequencing analysis to evaluate the role of lactoferrin in the LPS-induced acute inflammation in mouse Methods: 4 hs after intraperitoneal injection with LPS(4mg/kg) or equal volume PBS , the peritoneal cells were isolated from the peritoneal lavage fluid from wild type and lactoferrin knockout mice (n=3 for each group) . Then the cell RNA was sequenced by the HiSeq 2500 high-throughput sequencing service (Oebiotech, Shanghai, China). Data were extracted and normalized according to the manufacturer’s standard protocol. Results: Log-fold changes of up- or down-regulated mRNAs between the control and experiment group were selected with a significance threshold of p<0.05. Conclusions: Our study describes the mRNA expression profile changes of peritoneal cells from wild type and lactoferrin knockout mice during LPS-induced acute inflammation

2022-06-21 | GSE181752 | GEO

Transcriptome analysis reveals the mechanism of bovine lactoferrin against lipopolysaccharide-induced acute lung injury

Project description:Lactoferrin (LF) is an iron-binding multifunctional cationic glycoprotein secreted by exocrine glands and neutrophils in mammals. In this study, we created a mouse model of lipopolysaccharide (LPS)-induced acute lung injury and explored the anti-inflammatory effect and mechanism of bovine lactoferrin (bLF) in acute lung injury using the RNA sequencing (RNA-seq) technology and transcriptome analysis.

2023-12-19 | GSE247266 | GEO

MOUSE peritoneal macrophages treated with C498 or LPS

Project description:Mouse peritoneal macrophages were isolated and treated with vehicle or C498 (15 μM) for 0.5 h, followed by LPS (100 ng/ml) challenge for an additional 4 h.

2023-05-03 | GSE220654 | GEO

miRNA profiling in mouse peritoneal macrophages treated without or with LPS

Project description:We have identified a number of miRNAs that are differentially expressed in LPS treated mouse peritoneal macrophages, as compared to untreated cells

2014-02-28 | GSE55414 | GEO

miRNA profiling in mouse peritoneal macrophages treated without or with LPS

Project description:We have identified a number of miRNAs that are differentially expressed in LPS treated mouse peritoneal macrophages, as compared to untreated cells Peritoneal macrophages treated with LPS for 0, 4, 12, 24h. RNA was isolated and miRNA array assays were performed by Exiqon (miRCURY™ LNA Array version 10.0)

2014-02-28 | E-GEOD-55414 | biostudies-arrayexpress

Effect of lactoferrin treatment on iAEC2

Project description:In an attempt to understand the mode of antiviral action of lactoferrin on SARS-CoV-2 infection, we focused on the effect of lactoferrin on uninfected cells. We decided to use a physiologically relevant cell model of iPSC derived- alveolar epithelial cells (the iAEC2). We specifically focused on antiviral pathway triggered by lactoferrin on treated cells that could explain a cell-mediated effect of lactoferrin on viral replication

2021-07-15 | GSE171390 | GEO

Mouse peritoneal macrophages treated with LPS for 6 hours

Project description:Mouse peritoneal macrophages treated with LPS for 6 hours

| PRJNA697935 | ENA

Effects of lactoferrin, sophorolipids and lactoferrin-sophorolipids-assembly on gene expression of human dermal fibroblasts (HDFn)

Project description:The aim of this study is investigate effects of lactoferrin on human dermal fibroblasts and if sophorolipids influence effects of lactoferrin on human dermal fibroblasts.

2016-07-08 | GSE84144 | GEO

Transcriptional responses of mouse BMM and TEPM to lipopolysaccharide (LPS)

Project description:Evolutionary change in gene expression is generally considered to be a major driver of phenotypic differences between species. We investigated innate immune diversification by analyzing inter-species differences in the transcriptional responses of primary human and mouse macrophages to the TLR4 agonist, LPS. Using a custom platform permitting cross-species interrogation coupled with deep sequencing of mRNA 5M-bM-^@M-^Y ends, we identified extensive divergence in LPS-regulated orthologous gene expression between humans and mice (24% of orthologs, http://www.macgate.qfab.org). Divergently regulated (DR) orthologs were enriched for genes encoding cellular M-bM-^@M-^\inputsM-bM-^@M-^] such as cell surface receptors (e.g. TLR6, IL-7RM-NM-1), and functional M-bM-^@M-^\outputsM-bM-^@M-^] such as inflammatory cytokines/chemokines (e.g. CCL20, CXCL13). Conversely, intracellular signaling components linking inputs to outputs were typically concordantly regulated. DR genes were associated with a large dynamic range of gene expression, and specific promoter architectural features (TATA box enrichment, CpG island depletion). Surprisingly, regulatory divergence was also associated with enhanced inter-species promoter conservation. Thus, the genes controlled by complex, highly conserved promoters that facilitate dynamic regulation are also the most susceptible to evolutionary change. Mouse macrophages (bone marrow-derived macrophages, BMM and thioglycollate-elicited peritoneal macrophages, TEPM) were stimulated with the TLR4 agonist, lipopolysaccharide, over a time course (0, 2, 6, 24h) and analysed in biological triplicate on a custom-designed, focused microarray.

2012-03-28 | E-GEOD-19490 | biostudies-arrayexpress

Spleen vs Lipopolysaccharide (LPS) Treated B cell

Project description:total RNA from mouse (male c57BL/6) spleen labeled with Cy3 vs total RNA from mouse (male c57BL/6) B cells treated with Lipopolysaccharide (LPS) labeled with Cy5- time course with repeats Keywords: ordered

2003-04-14 | GSE389 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data