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Ezh2 is essential for the generation of functional yolk sac derived erythro-myeloid progenitors.

ABSTRACT: Ezh2 is essential for the generation of functional yolk sac derived erythro-myeloid progenitors.

PROVIDER: PRJNA753778 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR11770400_1.fastq.gz	Fastqsanger.gz
	SRR11770400_2.fastq.gz	Fastqsanger.gz
	SRR11770401_1.fastq.gz	Fastqsanger.gz
	SRR11770401_2.fastq.gz	Fastqsanger.gz
	SRR11770402_1.fastq.gz	Fastqsanger.gz

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Ezh2 is essential for the generation of functional yolk sac derived erythro-myeloid progenitors [RNA-seq]

Project description:Yolk sac (YS) hematopoiesis is critical for the survival of the embryo and a major source of tissue-resident macrophages that persist into adulthood. Here we report that the epigenetic regulator Ezh2 is essential for YS hematopoiesis but dispensable for subsequent aorta–gonad–mesonephros (AGM) blood development. Loss of Ezh2 activity in hemogenic endothelium (HE) leads to the generation of phenotypically intact but functionally deficient erythro-myeloid progenitors (EMP). Ezh2 activity is critical at the onset of the endothelial-to-hematopoietic transition generating EMPs but rapidly dispensable after that. We identified a lack of downregulation of Wnt signaling as the primary reason in the generation of non-functional EMPs. Together, our findings demonstrate a critical and specific role of Ezh2 in modulating Wnt signaling during the generation of EMPs from YS HE.

2021-08-16 | GSE150032 | GEO

Ezh2 is essential for the generation of functional yolk sac derived erythro-myeloid progenitors [ATAC-seq]

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Ezh2 is essential for the generation of functional yolk sac derived erythro-myeloid progenitor [CUT&RUN]

2021-08-16 | GSE181869 | GEO

Ezh2 is essential for the generation of functional yolk sac derived erythro-myeloid progenitors [ATAC-seq]

Project description:Ezh2 is essential for the generation of functional yolk sac derived erythro-myeloid progenitors [ATAC-seq]

| PRJNA631726 | ENA

Ezh2 is essential for the generation of functional yolk sac derived erythro-myeloid progenitors [RNA-seq]

Project description:Ezh2 is essential for the generation of functional yolk sac derived erythro-myeloid progenitors [RNA-seq]

| PRJNA630969 | ENA

Ezh2 is essential for the generation of functional yolk sac derived erythro-myeloid progenitor [CUT&RUN]

Project description:Ezh2 is essential for the generation of functional yolk sac derived erythro-myeloid progenitor [CUT&RUN]

| PRJNA753786 | ENA

The role of the yolk sac in human fetal development and identification of a hepatocyte-like cell in the human yolk sac

Project description:The human yolk sac (YS) is an extra-embryonic tissue critical for early prenatal life development. It is the first site of haematopoiesis where progenitors differentiate from endoderm within blood islands of the yolk sac contributing initially to primitive erythropoiesis and in subsequent waves to erythro-myeloid and lymphoid differentiation.

2023-03-01 | E-MTAB-10552 | biostudies-arrayexpress

The role of the yolk sac in human fetal development and identification of a hepatocyte-like cell in the human yolk sac (SS2)

2023-03-01 | E-MTAB-10888 | biostudies-arrayexpress

Ezh2 is essential for the generation of functional yolk sac derived erythro-myeloid progenitors.

Project description:Yolk sac (YS) hematopoiesis is critical for the survival of the embryo and a major source of tissue-resident macrophages that persist into adulthood. Yet, the transcriptional and epigenetic regulation of YS hematopoiesis remains poorly characterized. Here we report that the epigenetic regulator Ezh2 is essential for YS hematopoiesis but dispensable for subsequent aorta-gonad-mesonephros (AGM) blood development. Loss of EZH2 activity in hemogenic endothelium (HE) leads to the generation of phenotypically intact but functionally deficient erythro-myeloid progenitors (EMPs), while the generation of primitive erythroid cells is not affected. EZH2 activity is critical for the generation of functional EMPs at the onset of the endothelial-to-hematopoietic transition but subsequently dispensable. We identify a lack of Wnt signaling downregulation as the primary reason for the production of non-functional EMPs. Together, our findings demonstrate a critical and stage-specific role of Ezh2 in modulating Wnt signaling during the generation of EMPs from YS HE.

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Single-cell sequencing of yolk sac and fetal liver hematopoietic progenitors

Project description:Erythro-myeloid progenitors (EMP) and their progeny were labeled with YFP in mouse embryos using genetic fate mapping (constitutive Csf1r-iCre;Rosa26-eYFP or inducible Csf1r-MeriCreMer;Rosa26-eYFP injected at embryonic day E8.5 with 4-hydroxytamoxifen). EMP-derived progenitors (Lin- Kit+ YFP+) were sorted by FACS for single-cell transcriptomic analysis using the MARS-Seq approach. The purpose was to uncover heterogeneity and differentiation trajectories of EMP by comparing their transcriptional status at different developmental timepoints (E9.5, E10.5 and E12.5) and across niches (yolk sac and fetal liver).

2021-02-05 | GSE166223 | GEO

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