Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Single-cell dissection of Comma-1D cultures reveals cell populations with unique differentiation and transformation properties

ABSTRACT: Single-cell dissection of Comma-1D cultures reveals cell populations with unique differentiation and transformation properties

PROVIDER: PRJNA755982 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other
	SRR15510505_1.fastq.gz	Fastqsanger.gz
	SRR15510505_2.fastq.gz	Fastqsanger.gz
	SRR15510506_1.fastq.gz	Fastqsanger.gz
	SRR15510506_2.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 37

Similar Datasets

Single-cell dissection of Comma-1D cultures reveals cell populations with unique differentiation and transformation properties [scRNA-Seq]

Project description:The mammary epithelial tree is a heterogeneous structure that contains two main populations, luminal and basal, with distinct molecular and functional characteristics. The investigation of how this heterogeneity is developed and how it influences breast tumorigenesis has been hampered by the need to perform these studies using animal models due to the lack of relevant, tractable, and fully characterized in vitro models. Comma-1D is an immortalized mouse mammary epithelial cell line that has unique morphogenetic properties particularly its ability to generate cells of luminal and basal lineages. Here, we perform single-cell RNA-seq studies to dissect its heterogeneous nature. Our analysis reveals that Comma-1D cultures consist of two main populations with luminal and basal features and a smaller population with mixed lineage and bipotent characteristics. Additionally, we demonstrate that multiple transcription factors associated with the differentiation of the mammary epithelium in vivo also modulate this process in Comma-1D cultures. Finally, we have used the Comma-1D model to study how the cellular subtype of origin influences the response to cancer-promoting mutations. We found that only cells with luminal molecular features were able to acquire transformed characteristics after an oncogenic HER2 mutant was introduced in their genomes. Overall, the studies present here characterize at a single-cell level the heterogeneity of the Comma-1D cell line and illustrate how the unique properties of Comma-1D cells can be used as an experimental model to study both the differentiation and the transformation processes in vitro.

2022-05-04 | GSE182352 | GEO

Single-cell dissection of Comma-1D cultures reveals cell populations with unique differentiation and transformation properties [bulk RNA-Seq]

2022-05-04 | GSE182351 | GEO

Single-cell dissection of Comma-1D cultures reveals cell populations with unique differentiation and transformation properties [scRNA-Seq]

Project description:Single-cell dissection of Comma-1D cultures reveals cell populations with unique differentiation and transformation properties [scRNA-Seq]

| PRJNA755989 | ENA

Single-cell dissection of Comma-1D cultures reveals cell populations with unique differentiation and transformation properties [bulk RNA-Seq]

Project description:Single-cell dissection of Comma-1D cultures reveals cell populations with unique differentiation and transformation properties [bulk RNA-Seq]

| PRJNA755988 | ENA

Single-cell dissection of Comma-1D cultures reveals cell populations with unique differentiation and transformation properties

Project description:This SuperSeries is composed of the SubSeries listed below.

2022-05-04 | GSE182354 | GEO

Leveraging single cell transcriptomics and custom high throughput functional screening assays to resolve cell type, growth kinetic, and stemness heterogeneity within Comma-1D

Project description:We investigated the heterogeneous cell populations within the Comma-1D cell line through single cell RNA sequencing technologies. The assay sequenced over 5,000 cells composing 8 unique subclusters. Populations detected included luminal, myoepithelial, and fibroblast like cell types.

2021-12-31 | GSE182589 | GEO

Genome-wide expression analysis of bri1-5 and its suppressors brs1-1D, bak1-1D, bri1-1D

Project description:bri1-5 is a weak mutant of Brassinosteroid Insensitive 1 (BRI1). Suppressors by activation tagging bri1-1D, brs1-1D and bak1-1D can recover bri1-5 phenotype. We use microarray to investigate which pathways or functional categories have been transcriptionally regulated by bri1-1D, brs1-1D and bak1-1D.

2015-07-14 | GSE70843 | GEO

Hesperia comma

Project description:Hesperia comma overview

| PRJNA724778 | ENA

Leucania comma

Project description:Leucania comma overview

| PRJNA990903 | ENA

Cyclin D1, Id2 and Sox9 are upregulated in a lobular competent COMMA Db clonal derivative’ cells compared to a lobular incompetent clone both in vitro, and in vivo

Project description:Understanding underlying mechanisms responsible for the differentiation and stemness of mouse mammary progenitor cells are paramount to understanding the heterogeneity of mammary development and tumorigenesis. Consequently, studies have isolated and characterized mouse mammary progenitor cell lines such as: COMMA-1D, NSP2 and SP3, but have not parsed the various transcriptomes that play a role in progenitor differentiation and mammary gland development.

2018-10-19 | GSE121499 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data