Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

V. cholerae transcriptome without treatment and with indole or sub-MIC tobramycin

ABSTRACT: V. cholerae transcriptome without treatment and with indole or sub-MIC tobramycin

PROVIDER: PRJNA756892 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR15564521.fastq.gz	Fastqsanger.gz
	SRR15564522.fastq.gz	Fastqsanger.gz
	SRR15564523.fastq.gz	Fastqsanger.gz
	SRR15564524.fastq.gz	Fastqsanger.gz
	SRR15564525.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 9

Similar Datasets

V. cholerae transcriptome without treatment and with indole or sub-MIC tobramycin

Project description:We study gene expression levels of V. cholerae N16961 hapR+ corrected strain in exponential phase without and with indole or tobramycin.

2021-08-26 | GSE182561 | GEO

Effect of sub-MIC tobramycin treatment on E. coli MG1655 WT and NCM3416

Project description:Effect of sub-MIC tobramycin treatment on E. coli MG1655 WT (strain classically used as WT strain) and NCM3416 (corrected rph strain). Comparison of genes expression without treatment (MH) in E. coli MG1655 WT and NCM3416.

2023-03-01 | GSE224240 | GEO

Effect of sub-MIC tobramycin treatment on E. coli MG1655 WT and NCM3416

Project description:Effect of sub-MIC tobramycin treatment on E. coli MG1655 WT and NCM3416

| PRJNA930389 | ENA

V. cholerae transcriptome or deleted for the tgt gene in MH, supplemented or not with tobramycin or ciprofloxacin

Project description:We study gene expression levels of V. cholerae N16961 WT and tgt mutant strains in MH medium in the presence or not of sublethal concentration on tobramycin or ciprofloxacin

2022-10-03 | GSE214520 | GEO

Kristen-PAO1 aerobic sub-inhibitory cond. tobramycin

Project description:To see the effect of sub-inhibitory concentrations of tobramycin on Pseudomonas aeruginosa grown under aerobic conditions. RNA was isolated form 4 biological repeats of P.aeruginosa grown to mid-log in cationic adjusted mueller hinton broth and 4 biological repeats of P.aeruginosa with the addition of 0.25ug/ml tobramycin in cationic adjusted mueller hinton broth. null

2011-03-30 | E-FPMI-23 | biostudies-arrayexpress

Transposon Insertion Sequencing to study the genetic interactions between V. cholerae WT cells and two mutant strains in absence or presence of subinhibitory doses of tobramycin

Project description:This study was conducted to study which genes or functions become important or expendable in two V. cholerae mutants compared to the WT. This was assessed directly by conjugating a Mariner Transposon with WT or mutant cells and obtaining a bank of hundreds of thousands of mutants which we consider as the T=0. We then subjected these banks to 16 generations in rich media with or without 50% MIC of tobramycin (which we call T=16gen). At both T0 and T16gen we extracted the genomic DNA of these banks and sequenced it to identify the regions where the transposon is inserted in each strain at the different conditions.

2023-04-26 | GSE198341 | GEO

Kristen-PAO1-anaerobic sub-lethal concentrations tobramycin

Project description:To see the effect of sub-lethal concentration of tobramcyin on PAO1 under anaerobic conditions. RNA was isolated from 5 biological repeats of PAO1 grown to mid-log phase in cationic adjusted mueller hinton broth containing 15mM KNO3 and 5 biological repeats of PAO1 grown to mid-log phase in cationic adjusted mueller hinton broth containing 15mM KNO3 and then treated for 30 minutes with 2 ug/ml tobramycin. All flasks were sealed to create anaerobic conditions. null

2011-01-20 | E-FPMI-25 | biostudies-arrayexpress

Analysis of antibiotic response in Clinical Wound Pseudomonas aeruginosa isolates: Unveiling Proteome Dynamics of tobramycin tolerant phenotype

Project description:Pseudomonas aeruginosa (PA) is an opportunistic human pathogen, causing serious chronic infections. PA can adapt efficiently to antibiotic stressors via different genotypic or phenotypic strategies such as resistance and tolerance. The adaptation regulatory system is not always very well understood. In this study, we use shotgun proteomics to investigate the system-level response to tobramycin in two clinical wound PA isolates and PAO1. We profiled each strain for its antibiotic drug-tolerant phenotype using supra-minimum inhibitory concentrations (supra-MIC) of tobramycin and apply proteomics to investigate the protein expression profiles. The MIC revealed that all isolates were susceptible to tobramycin but at supra-MIC concentrations at stationary growth, a degree of tolerance was observed for the isolates. We identified around 40 % of the total proteins encoded by the PA genome and highlighted shared and unique protein signatures for all isolates. Comparative proteome profiling in the absence of antibiotic treatment showed divergent fingerprints, despite similarities in the growth behavior of the isolates. In the presence of tobramycin, the isolates shared a common response in the downregulation of proteins involved in the two-component system, whereas stress response proteins were present at higher levels. Our findings provide insight into the use of proteomic tools to dissect the system-level response in clinical isolates in the absence and presence of antibiotic stress.

2024-10-17 | PXD051528 | Pride

Tobramycin Treatment of Planktonic Pseudomonas aeruginosa

Project description:As a comparison to tobramycin-treated P. aeruginosa biofilms, we investigated the response of planktonic P. aeruginosa to tobramycin by microarray. Keywords: Tobramycin Response

2008-06-10 | GSE9991 | GEO

Whole genome sequencing of sub-MIC tunicamycin adaptors in Cryptococcus neoformans

Project description:Whole genome sequencing of sub-MIC tunicamycin adaptors in Cryptococcus neoformans

| PRJEB56625 | ENA