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Transposon Insertion Sequencing to study the genetic interactions between V. cholerae WT cells and two mutant strains in absence or presence of subinhibitory doses of tobramycin

ABSTRACT: This study was conducted to study which genes or functions become important or expendable in two V. cholerae mutants compared to the WT. This was assessed directly by conjugating a Mariner Transposon with WT or mutant cells and obtaining a bank of hundreds of thousands of mutants which we consider as the T=0. We then subjected these banks to 16 generations in rich media with or without 50% MIC of tobramycin (which we call T=16gen). At both T0 and T16gen we extracted the genomic DNA of these banks and sequenced it to identify the regions where the transposon is inserted in each strain at the different conditions.

ORGANISM(S): Vibrio cholerae O1 biovar El Tor str. N16961

PROVIDER: GSE198341 | GEO | 2023/04/26

REPOSITORIES: GEO

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