RNA-seq from ileal lamina propria CD4+ cell transcripts in germ-free and E. lenta monocolonized mice
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA757751 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR15611782.fastq.gz | Fastqsanger.gz | |||
| SRR15611783.fastq.gz | Fastqsanger.gz | |||
| SRR15611784.fastq.gz | Fastqsanger.gz | |||
| SRR15611785.fastq.gz | Fastqsanger.gz | |||
| SRR15611786.fastq.gz | Fastqsanger.gz |
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Project description:The goals of this study were to assess the differentially expressed transcripts in CD4+ cell isolated from the ileal lamina propria of germ-free (GF) and E. lenta strain 2243 monocolonized mice.
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Project description:To further development of our gene expression approach to CD300a deficiency on dendritic cells (DCs) in colonic lamina propria, we have employed whole genome microarray expression profiling as a discovery platform to identify genes with the potential to distinguish CD300a deficiency on DCs in colonic lamina propria from those of WT mice. Colonic lamina propria DCs were obtained by cell sorter from WT and CD300a deficient mice raised under SPF and GF condition. Expression of Ifnb1 was significantly higher in CD300a deficient DCs, quantified in the same RNA samples by real-time PCR. Gene expression in WT and CD300a colonic lamina propria DCs raised under SPF and GF conditions were measured. Colonic lamina propria cells were obtained from 5 mice in each conditions. Takara-Bio
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