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Single-cell RNA sequencing of self-maintaining lamina propria macrophages

ABSTRACT: Single-cell RNA sequencing of 697 YFP+ CD64+ lamina propria macrophages isolated from Cx3cr1CreERT2.Rosa26-LSL-YFP mice 35 weeks after tamoxifen administration

INSTRUMENT(S): Illumina HiSeq 4000

ORGANISM(S): Mus musculus

SUBMITTER: Sebastiaan De Schepper

PROVIDER: E-MTAB-6976 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Self-Maintaining Gut Macrophages Are Essential for Intestinal Homeostasis.

De Schepper Sebastiaan S Verheijden Simon S Aguilera-Lizarraga Javier J Viola Maria Francesca MF Boesmans Werend W Stakenborg Nathalie N Voytyuk Iryna I Schmidt Inga I Boeckx Bram B Dierckx de Casterlé Isabelle I Baekelandt Veerle V Gonzalez Dominguez Erika E Mack Matthias M Depoortere Inge I De Strooper Bart B Sprangers Ben B Himmelreich Uwe U Soenen Stefaan S Guilliams Martin M Vanden Berghe Pieter P Jones Elizabeth E Lambrechts Diether D Boeckxstaens Guy G

Cell 20180830 2

Macrophages are highly heterogeneous tissue-resident immune cells that perform a variety of tissue-supportive functions. The current paradigm dictates that intestinal macrophages are continuously replaced by incoming monocytes that acquire a pro-inflammatory or tissue-protective signature. Here, we identify a self-maintaining population of macrophages that arise from both embryonic precursors and adult bone marrow-derived monocytes and persists throughout adulthood. Gene expression and imaging s ...[more]

PMID: 30173915

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