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Regulation of group 3 innate lymphoid cells by Setd2 [RNAseqILC3]

ABSTRACT: Regulation of group 3 innate lymphoid cells by Setd2 [RNAseqILC3]

PROVIDER: PRJNA758044 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR15621980_1.fastq.gz	Fastqsanger.gz
	SRR15621980_2.fastq.gz	Fastqsanger.gz
	SRR15621981_1.fastq.gz	Fastqsanger.gz
	SRR15621981_2.fastq.gz	Fastqsanger.gz
	SRR15621982_1.fastq.gz	Fastqsanger.gz

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Regulation of group 3 innate lymphoid cells by Setd2

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Project description:To determine the molecular regulation of different subsets of ILC3s by Setd2, small intestinal NKp46- ILC3s (Thy1highCD45low NKp46-) and NKp46+ ILC3s (Thy1highCD45low NKp46+) from Setd2-deficient and control mice were subjected to assay for RNA-seq.

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Project description:This SuperSeries is composed of the SubSeries listed below. To determine the molecular regulation of different subsets of ILC3s by Setd2, small intestinal NKp46- ILC3s (Thy1highCD45low NKp46-) and NKp46+ ILC3s (Thy1highCD45low NKp46+) were purified from littermate Rag1-/-;Setd2f/f or Rag1-/-;Rorc-cre;Setd2f/f mice. Cells were subjected to RNA-seq and ATAC-seq analyses.

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Project description:We analyzed the total proteome of group 2 innate lymphoid cells (ILC2s) after different stimulation with interleukin-33 (IL-33), a cytokine playing a critical role in human asthma, and TL1A, a TNF-family cytokine also known to activate ILC2s. Upon combined stimulation with IL-33 plus TL1A, we show that lung ILC2s produce high amounts of IL-9 and acquire a transient ‘ILC9’ phenotype. This phenotype is characterized by simultaneous production of large amounts of type 2 cytokines (IL-5, IL-13 and IL-9), induction of the IL-2 receptor CD25 (Il2ra), and of the transcription factors IRF4, JunB and BATF, that form immune-specific complexes known to induce IL-9 expression.

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