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ABSTRACT: Histological and proteotranscriptomic characterisation of byssus components in the pearl oyster, Pinctada maxima

PROVIDER: PRJNA761869 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR15827334_1.fastq.gz	Fastqsanger.gz
	SRR15827334_2.fastq.gz	Fastqsanger.gz
	SRR15827335_1.fastq.gz	Fastqsanger.gz
	SRR15827335_2.fastq.gz	Fastqsanger.gz
	SRR15827336_1.fastq.gz	Fastqsanger.gz

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Global gene expression profile for pearl oyster

Project description:This SuperSeries is composed of the following subset Series: GSE13980: Analysis of the global gene expression profile for pearl oyster, Pinctada maxima, exposed to organotin (tributyltin) GSE14303: Differential expression analysis of genes from the mantle tissue of pearl oyster: Pinctada maxima GSE14305: The microstructural, mineralogical and transcriptional developments of shell biomineralization of Pinctada maxima Refer to individual Series

2010-02-04 | E-GEOD-14306 | biostudies-arrayexpress

Proteomic characterisation of byssus components in the pearl oyster, Pinctada maxima

Project description:Many bivalve species produce groups of strong proteinaceous byssal threads to rigidly attach to underwater substrates. Fibres like these have potential applications as biomedical materials due to their unique mechanical characteristics. The byssus and byssal thread producing glands of Pinctada maxima have not yet been characterised. RNA was isolated from P. maxima foot and byssal stem region tissues and sequenced using the Illumina platform. A de novo reference transcriptome comprising 34,281 contiguous sequences was assembled, and tissue replicates were mapped against the reference for quantitative analysis. Tryptic digests of byssal threads were analysed by LC-MS/MS. The resultant peptides were matched to 62 protein sequences derived from our reference transcriptome. Components of the byssus were identified for further characterisation, including a highly expressed perlucin-like foot protein (Pmfp1) and a recently identified protein that we refer to herein as glycine-rich thread (GRT) protein. This work provides principal knowledge on the molecular components of the byssus for P. maxima and the foot ultrastructure involved in the creation of byssal threads. This study advances our knowledge of byssus biosynthesis in non-mytilids, providing a platform for the design of new marine biopolymers.

2022-07-31 | PXD028273 | Pride

miRNA expression profile of mantle tissues for pearl oyster, Pinctada fucata

Project description:To elucidate the modulatory participation of miRNAs in mollusk biomineralization, we have employed high-throughput sequencing to identify miRNAs of pearl oyster, Pinctada fucata. Our study focused on the miRNA expression profile of the mantle, an organ responsible for shell formation of the oyster. The pearl oysters were cultured in the tank with the maintaining conditions of temperature 19 ℃, PH 8.1 and salinity 33‰ in recirculating seawater.

2021-05-01 | GSE145993 | GEO

Project description:Intestinal microbial community of pearl oyster Pinctada maxima

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cDNA microarray analysis revealing candidate biomineralizational genes of the pearl oyster, Pinctada fucata martensii

Project description:In order to screen and identify biomineralization gene, microarray technique was used to reveal tissues specific expression genes in the brunet mantle edge (ME), mantle centre (MC), and both ME and MC (ME-MC) from assembled transcriptome contigs of Pinctada fucata martensii, ideal pearl oyster for the study of biomineralization. Tissues of ME, MC, hepatopancreas, foot, gill, adductor muscle, heart and intestine were sampled from two females and one male pearl oyster. Gonad was sampled from above three individuals and another two male and one female. Equal amount RNA of each individual hepatopancreas, foot, gill, adductor muscle, heart and intestine were mixed as a composite viscera sample (CV), and equal amount gonad RNA from one male and one female were combined together as a gonad sample (GS). Hybridizations were performed with twelve samples of ME, MC, CV and GS. Gene expression in ME, MC, gonad and other tissues were measured. Gonads were sampled from 6 individuals, and other tissues were sampled from above three individuals.

2016-07-01 | E-GEOD-70439 | biostudies-arrayexpress

cDNA microarray analysis revealing candidate biomineralizational genes of the pearl oyster, Pinctada fucata martensii

2016-07-01 | GSE70439 | GEO

Proteomic analysis on different tissues of Pteria penguin

Project description:The winged pearl oyster, Pteria penguin, is a widely distributed and economically important bivalve with unique and primitive byssus. The byssogensis of this species is largely unknown. Here we report the lable free quantification analysis on different tissues of P. penguin.Through the analysis, we found that the process of byssogenesis requires ATP to provide energy among the differential proteins; and seven (Tenascin-X, laminin, papilin, fibrillin-1, spondin, protease inhibitors and Mucins) of the 31 proteins identified in the byssus were derived from ECM. Functions of extracellular matrix proteins can be correlated with the formation and the nature of the byssus.

2023-12-20 | PXD031701 | Pride

Comparative proteomics reveals the immune reaction of the pearl oyster Pinctada fucata serum to xenograft from Pinctada maxima, the pearl oyster, allograft transplantation, xenograft transplantation, Immune rejection, comparative proteomics, pearl

Project description:To determine the mechanism underlying the immune response after allograft (mantle grafts of Pinctada fucata) and xenograft (mantle pieces of Pinctada maxima) transplantations in the pearl oyster Pinctada fucata, two sets of serum were obtained at different times (6, 12, 24, 48, 96, 144 and 192 h) after allograft and xenograft transplantations and proteomic responses were evaluated by using isobaric tags for relative and absolute quantification labeling coupled with liquid chromatography (LC) / tandem mass spectrometric (MS) analysis.

2023-08-24 | PXD044839 |

Pinctada fucata martensii shell matrix protein LC-MSMS

Project description:Nacre, the iridescent material found in pearls and shells of molluscs, is formed through an extraordinary process of matrix-assisted biomineralization. Despite recent advances, many parts of the biomineralization process and its evolutionary origin remain a mystery. The pearl oyster Pinctada fucata martensii is a well-known master of biomineralization, but the molecular mechanisms underlie its production of remarkable shells and pearls is not fully understood. We sequenced the highly polymorphic genome of the pearl oyster and conducted multi-omic and biochemical studies to probe nacre formation. We identified a large set of novel proteins participating in matrix-framework formation, many in expanded families, including components similar to that found in vertebrate bones such as collagen-related VWA-containing proteins (VWAP), chondroitin sulfotransferases and regulatory elements.Considering that there are only collagen-based matrices in vertebrate bones and chitin-based matrices in most invertebrate skeletons, the presence of both chitin and elements of collagen-based matrices in nacre matrices suggests that elements of chitin- and collagen-based matrices are deeply rooted and might be part of an ancient biomineralizing matrix. Our results expand the current shell matrix-framework model and provide new insights into the evolution of diverse biomineralization systems.

2017-07-07 | PXD006786 | Pride

Project description:Pinctada maxima mantle and pearl sac transcriptomes

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