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ABSTRACT: Intestinal microbial community of pearl oyster Pinctada maxima

PROVIDER: PRJNA774900 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR16605509.fastq.gz	Fastqsanger.gz
	SRR16605510.fastq.gz	Fastqsanger.gz
	SRR16605511.fastq.gz	Fastqsanger.gz
	SRR16605512.fastq.gz	Fastqsanger.gz

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Global gene expression profile for pearl oyster

Project description:This SuperSeries is composed of the following subset Series: GSE13980: Analysis of the global gene expression profile for pearl oyster, Pinctada maxima, exposed to organotin (tributyltin) GSE14303: Differential expression analysis of genes from the mantle tissue of pearl oyster: Pinctada maxima GSE14305: The microstructural, mineralogical and transcriptional developments of shell biomineralization of Pinctada maxima Refer to individual Series

2010-02-04 | E-GEOD-14306 | biostudies-arrayexpress

miRNA expression profile of mantle tissues for pearl oyster, Pinctada fucata

Project description:To elucidate the modulatory participation of miRNAs in mollusk biomineralization, we have employed high-throughput sequencing to identify miRNAs of pearl oyster, Pinctada fucata. Our study focused on the miRNA expression profile of the mantle, an organ responsible for shell formation of the oyster. The pearl oysters were cultured in the tank with the maintaining conditions of temperature 19 ℃, PH 8.1 and salinity 33‰ in recirculating seawater.

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cDNA microarray analysis revealing candidate biomineralizational genes of the pearl oyster, Pinctada fucata martensii

Project description:In order to screen and identify biomineralization gene, microarray technique was used to reveal tissues specific expression genes in the brunet mantle edge (ME), mantle centre (MC), and both ME and MC (ME-MC) from assembled transcriptome contigs of Pinctada fucata martensii, ideal pearl oyster for the study of biomineralization. Tissues of ME, MC, hepatopancreas, foot, gill, adductor muscle, heart and intestine were sampled from two females and one male pearl oyster. Gonad was sampled from above three individuals and another two male and one female. Equal amount RNA of each individual hepatopancreas, foot, gill, adductor muscle, heart and intestine were mixed as a composite viscera sample (CV), and equal amount gonad RNA from one male and one female were combined together as a gonad sample (GS). Hybridizations were performed with twelve samples of ME, MC, CV and GS. Gene expression in ME, MC, gonad and other tissues were measured. Gonads were sampled from 6 individuals, and other tissues were sampled from above three individuals.

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Comparative proteomics reveals the immune reaction of the pearl oyster Pinctada fucata serum to xenograft from Pinctada maxima, the pearl oyster, allograft transplantation, xenograft transplantation, Immune rejection, comparative proteomics, pearl

Project description:To determine the mechanism underlying the immune response after allograft (mantle grafts of Pinctada fucata) and xenograft (mantle pieces of Pinctada maxima) transplantations in the pearl oyster Pinctada fucata, two sets of serum were obtained at different times (6, 12, 24, 48, 96, 144 and 192 h) after allograft and xenograft transplantations and proteomic responses were evaluated by using isobaric tags for relative and absolute quantification labeling coupled with liquid chromatography (LC) / tandem mass spectrometric (MS) analysis.

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Project description:Histological and proteotranscriptomic characterisation of byssus components in the pearl oyster, Pinctada maxima

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