Project description:expression analysis in colon cancer from Msh2 and Tgfbr2 intestinal epithelium inactivated mouse model detailed gene expression analysis in colon cacer developed in mouse model with Msh2 and Tgfbr2 inactivated eary n from Villin Cre recombnase exclusively in the intestine
Project description:expression analysis in colon cancer from Msh2 and Tgfbr2 intestinal epithelium inactivated mouse model detailed gene expression analysis in colon cacer developed in mouse model with Msh2 and Tgfbr2 inactivated eary n from Villin Cre recombnase exclusively in the intestine
Project description:Goal of the experiment: To identify transcriptional patterns across tumors from colorectal cancer murine models and normal mouse colon samples at different developmental stages. Experiment description: Colorectal cancer (CRC) results from multiple genetic and epigenetic events that produce variable histologies and clinical outcomes. To identify gene regulatory programs that underlie colon tumorigenesis, we profiled gene expression in 39 mouse colon tumors from four independent mouse models and compared this to mouse colon embryonic development, as well as with 100 human colon carcinomas. Here, we report a striking recapitulation of embryonic patterns of gene expression in both mouse and human colon tumors. All four of the mouse colon tumor models exhibited large-scale activation of embryonic gene expression signatures. The two nuclear beta-catenin-positive mouse tumors (azoxymethane-treated [AOM] and ApcMin/+), exhibited strong activation of genes characteristic of those expressed in the earliest embryonic stages, while tumors from two other models (Smad3-/- and Tgfb1-/- x Rag2-/-) exhibited lower activation of early stage-specific genes but substantial expression of general embryonic colon genes. Human colon cancer cases over-expressed genes characteristic of both early and late embryonic stages. Examining tumor gene expression through the lens of development has revealed an extensive network of therapeutic targets for cancer control. Keywords: Tumors from four murine models of colorectal cancer and normal mouse colon samples at different developmental stages
Project description:DIGE results of mouse models of colon cancer. Tissue harvested from small intestines of Apc1638N+/- and p21-/- mice as per the method developed by Wieser et al. (1973). Spots identified as significant (alpha<0.05) in the comparison of mutant mouse crypts and villi to WT crypts and villi, respectively.
Project description:Functional genomics approach to metastatic colon cancer; Mouse model translated to human colon cancer Experiment Overall Design: Gene expression array differences between highly invasive mouse colon cancer cells and non-invasive colon cancer cells