Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Telomerase RNA subunit of Ustilago maydis

ABSTRACT: Telomerase RNA subunit of Ustilago maydis

PROVIDER: PRJNA762910 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR15880976_1.fastq.gz	Fastqsanger.gz
	SRR15880976_2.fastq.gz	Fastqsanger.gz
	SRR15880979_1.fastq.gz	Fastqsanger.gz
	SRR15880979_2.fastq.gz	Fastqsanger.gz
	SRR15880982_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 12

Similar Datasets

Telomerase RNA subunit of Ustilago maydis

Project description:The RNA subunit of telomerase is an essential component whose primary sequence and length are poorly conserved among eukaryotic organisms. The phytopathogen Ustilago maydis is a dimorphic fungus of the Ustilaginaceae family. We analyzed several members of the Ustilaginaceae family to computationally identify the TElomere RNA (TER) gene ter114. To confirm the identity of the TER gene, we disrupted the gene and characterized telomerase-negative mutants. Similar to catalytic TERT mutants, ter114Δ mutants exhibit phenotypes of telomere shortening and low replicative potential. However, ter114Δ mutants had very prolonged replication times, and survivors arose more rapidly in ter114Δ (252 replication rounds) than in their counterpart trt1Δ mutant strains (300 replication rounds). ter114-disrupted mutants were unable to infect maize seedlings in heterozygous crosses and showed defects such as cell cycle arrest and segregation failure. We concluded that ter114, which encodes the TER subunit of the telomerase of U. maydis, could have distinct or more extensive functions than trt1. To further characterize the ter114 gene, we analyze the transcriptomes of both, mRNA nuclear transcripts and nuclear RNA ribo-depleted transcripts of the wild-type strain 521, from the reads of both nuclear fractions, we find four reconstructed transcripts of the ter114 locus, which constitute four isoforms containing the structural domains of TER. The results showed that the transcription start site for the four isoforms is located upstream the start codon of the uncharacterized gene UMAG_03168, while the differential expression analysis showed that the ter114-i4 isoform is up-regulated with respect to the polyadenylated fraction, suggesting that this transcript could correspond to the precursor of the mature form of TER.

2021-09-17 | GSE184051 | GEO

Global gene expression of post-senescent telomerase negative ter1Δ strain of Ustilago maydis

Project description:The dimorphic fungus Ustilago maydis transits from unicellular sporidia to multicellular mycelium and accomplish its life cycle in planta with teliospore formation. Mutations in either of telomerase core subunits, trt1 or ter1 cause deficiencies or inability to produce galls and teliospores when crossed to a wild-type strain. Here, the global transcriptome analysis of two ter1Δ survivor strains of U. maydis is reported. It revealed the deregulation of many telomerase-deleted response (TDR) genes previously reported in other telomerase-negative fungi, as the DDR and stress response genes, cell cycle, subtelomeric and some telomere proximal genes. Interestingly, other differentially expressed genes (DEGs) found in those ter1Δ survivors were pathogenic lifestyle factors, plant-pathogen crosstalk, iron uptake, meiosis, and melanin synthesis. The studied survivors were phenotypically comparable, yet they exhibited DEGs between them. Our global results suggest the teliospore formation in U. maydis controlled by key genes of pathogenic lifestyle and meiosis

2023-09-04 | GSE225422 | GEO

mRNA profiling in Ustilago maydis under RUS (repopulation/regrowth under starvation)

Project description:mRNAs comparison between Ustilago maydis wild type grown in diluted YEPS (control) and in cell-free supernatants of Ustilago maydis wild type treated with H202 in two different concentrations (0.4% and 0.7%).

2022-06-16 | GSE205897 | GEO

Maize gene expression during infection with Ustilago maydis

2008-03-01 | GSE10023 | GEO

Transcriptomic analysis of basidiocarp development in Ustilago maydis (DC) Dcd

Project description:Study of gene regulation basidiocarps development in Ustilago maydis using transcriptomic analysis. In 2012, Cabrera-Ponce et al. established conditions allowing a completely different developmental program in U. maydis when grown on solid medium containing Dicamba (synthetic auxin) in dual cultures with maize embryogenic calli.

2017-12-31 | GSE89025 | GEO

Expression analysis of Pseudozyma antarctica T-34 and Ustilago maydis on different carbon source

Project description:Investigation of whole genome gene expression level in Pseudozyma antarctica T-34, compared to Ustilago maydis UM521. To clarify the transcriptomic characteristics of Pseudozyma antarctica under the conditions of high MEL production, a DNA microarray of both the strains, Pseudozyma antarctica T-34 and Ustilago maydis UM521 was prepared and analyzed the transcriptomes.

2014-03-21 | GSE47171 | GEO

Comparison of extracellular vesicle and filamentous cell transcriptomes of Ustilago maydis

Project description:The coding transcriptomes of filamentous cultures of the maize smut fungus Ustilago maydis and their extracellular vesicles (EVs) were compared. Protein-coding transcripts relatively enriched in EVs versus filament cells were identified and examined to identify potentially functional mRNA cargos of U. maydis EVs.

2021-06-08 | GSE176292 | GEO

Expression analysis of Pseudozyma antarctica T-34 and Ustilago maydis on different carbon source

2014-03-21 | E-GEOD-47171 | biostudies-arrayexpress

Transcription profiling of maizeduring infection with Ustilago maydis

Project description:The fungal pathogen Ustilago maydis establishes a biotrophic relationship with its host plant maize. Hallmarks of the disease are large plant tumors in which fungal proliferation occurs. Plants have developed various defense pathways to cope with pathogens. We used microarrays to detail the global programme of gene expression during the infection process of Ustilago maydis in its host plant to get insights into the defense programs and the metabolic reprogramming needed to supply the fungus with nutrients. Experiment Overall Design: In three independent experiments plants were infected with the solopathogenic U. maydis strain SG200. Samples from infected leaves were taken at 12 and 24 hours post infection, as well as 2, 4 and 8 days post infection. Samples from uninfected control plants were taken at the same time points.

2008-04-07 | E-GEOD-10023 | biostudies-arrayexpress

RNAseq of Ustilago maydis with inducible clp1 and Δcib1

Project description:Ustilago maydis strain JB1 was modified to include an arabinose inducible clp1 in the ip-locus and clb1 was deleted.

2019-04-04 | E-MTAB-7463 | biostudies-arrayexpress