Project description:To investigate how LXR activation potentiates expression of Th2 cytokine-dependent genes in primary human macrophages, we pulsed macrophages with synthetic LXR ligand T0901317 then polarized cells to alternatively activated macrophages with IL-4 or IL-13.
Project description:Experimental set 1: To evaluate the impact of apoptotic neutrophils on TLR4-induced inflammatory pathways in human macrophages. Experimental set 2: To compare the activation of inflammatory pathway between alveolar macrophages (AM) from idiopathic pulmonary fibrosis (IPF) patients and alveolar macrophages from respiratory bronchiolitis interstitial lung disease (RB-ILD) patients
Project description:Inflammation, mediated by liver-resident Kupffer cells (KCs) and recruited infiltrating macrophages (IMs), plays a critical role in NAFLD pathogenesis. Cholesterol induces pro-inflammatory cytokine production by macrophages and has been identified as a key factor involved in progression from simple steatosis to NASH. In this study, using the fructose, palmitate, cholesterol & trans-fat (FPC) diet model which recapitulates human NAFLD, we aimed to determine the impact of cholesterol manipulation on NAFLD progression/regression and macrophage phenotype in vivo.The transcriptomic evaluation of hepatic macrophages from mice fed with FPC diet contained the highest concentration of cholesterol (1.25%) suggest these cells might contribute to liver inflammation, steatohepatitis, and hepatocarcinoma pathways during the progression phase.
Project description:We investigate the impact of an initial stimulation and a re-stimulation in altering transcriptional responses in both peripheral blood-monocyte derived macrophages and human pluripotent stem cell derived macrophages.
