Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Whole transcriptome profiling of H37Rv- and H37Rv△1759c-infected macrophages.

ABSTRACT: Whole transcriptome profiling of H37Rv- and H37Rv△1759c-infected macrophages.

PROVIDER: PRJNA765598 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR16021464.fastq.gz	Fastqsanger.gz
	SRR16021466.fastq.gz	Fastqsanger.gz
	SRR16021468.fastq.gz	Fastqsanger.gz
	SRR16021470.fastq.gz	Fastqsanger.gz
	SRR16021472.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 9

Similar Datasets

Whole transcriptome profiling of H37Rv- and H37Rv△1759c-infected macrophages.

Project description:To explore the regulatory network of noncoding RNAs after M.tb infection and the role of Rv1759c in the infection process, we collected samples of H37Rv- and H37Rv△1759c-infected macrophages and explored the full transcriptome expression profile. We constructed DE-lncRNA/DE-circRNA-DE-miRNA-DE-mRNA regulatory networks during H37Rv and H37Rv△1759c infection. In addition, we first discovered the close relationship between Rv1759c and chemokines during M.tb infection by comparing the transcription profiles of H37Rv and H37Rv△1759c and bioinformatics analysis. Here, our study comprehensively characterizes the ncRNA and mRNA profiles in macrophages infected with H37Rv and H37Rv△1759c, which provides support and new directions for in-depth exploration of ncRNA and PE/PPE family functions during the infection process.

2021-09-30 | GSE184660 | GEO

miRNA profiles of Mtb H37Rv infected peritoneal macrophages and uninfected controls

Project description:The peritoneal macrophages were infected with Mtb H37Rv for 4 hours, and the miRNA expression profile were analyzed with deep sequencing.

2018-09-06 | GSE119495 | GEO

Comparative transcriptomic analysis of THP‑1 derived macrophages infected with Mycobacterium tuberculosis H37Rv, H37Ra and BCG

Project description:In this study, RNA sequencing (Transcriptome sequencing) was employed to investigate the global transcriptome changes in the macrophages during the different strains of M.tb infection. THP-1 cells derived macrophages were exposed to the virulent M.tb strain H37Rv (Rv) or the avirulent M.tb strain H37Ra (Ra), and the M.tb BCG vaccine strain was used as a control. The cDNA libraries were prepared from M.tb infected macrophages and then sequenced.

2020-12-06 | GSE162729 | GEO

miRNA profiles of Mtb H37Rv infected peritoneal macrophages and uninfected controls

Project description:miRNA profiles of Mtb H37Rv infected peritoneal macrophages and uninfected controls

| PRJNA489395 | ENA

RNA sequencing of uninfected, H37Rv- or Rv0222Ko-infected mouse peritoneal macrophages

Project description:RNA sequencing of uninfected, H37Rv- or Rv0222Ko-infected mouse peritoneal macrophages

| PRJNA558379 | ENA

Genome-wide maps of H3K4me1 in Mycobacterium tuberculosis H37Rv-infected macrophages

Project description:Genome-wide maps of H3K4me1 in Mycobacterium tuberculosis H37Rv-infected macrophages

| PRJEB8059 | ENA

TaqMan Human miRNA array profile of Mycobacterium tuberculosis H37Rv infected THP-1 differentiated macrophages cells.

Project description:Differentially regulated miRNA candidates in H37Rv infected THP-1 cells were analysed with respect to uninfected THP-1 reference samples. THP-1 cells are monocytes differentiated to macrophages after treatment with PMA for 48 hrs. Total RNA was isolated from infected THP-1 cells after 24 hrs of infection, cDNA was synthesized for TLDA real time PCR reaction using TaqMan MicroRNA Reverse Transcription kit and Megaplex Human Pool A and Pool B stem loop RT primers (version 3.0) as per manufacturer’s protocol. Further real time reaction was performed on QuantStudio 12K Flex Real-Time PCR System (Applied Biosystems) by using cDNA (without pre-amplification) on TLDA card A and card B (version 3.0).

2017-04-20 | GSE94007 | GEO

Transcriptome of M. tuberculosis-infected human macrophages in the presence of ATP

Project description:Human monocyte-derived macrophages were infected with Mycobacterium tuberculosis (H37Rv strain) at a multiplicity of infection 1:1. After 2h of incubation, cells were washed to remove extracellular bacteria and were incubated in fresh medium for a further 18h in the presence or not of 100microM ATP. Total RNA was then extracted and gene expression was determined using HuGene 1.0 ST microarrays (Affymetrix, Inc.,Santa Clara, CA).

2013-06-01 | E-MEXP-3805 | biostudies-arrayexpress

Real-time quantitative PCR analysis of PMA differentiated THP-1 infected with H37Rv or M. marinum, or heat killed H37Ra (HKMT)

Project description:THP-1 cells were differentiated into macrophages with 0,1 µM PMA overnight then infected for 2 days with either H37Rv, M. marinum or heat killed H37Ra. Then RNA was collected in order to quantify the inflammatory response transcription as compared with the relative uninfected controls.

2021-07-20 | GSE165327 | GEO

Vitamin D treatment of M.tb. infected macrophages

Project description:As macrophages are the primary site of Mtb infection and are sites of vitamin D signaling, we have used these cells to understand the molecular mechanisms underlying modulation of the immune response by the hormonal form of vitamin D, 1,25-dihydroxyvitamin D (1,25D). Microarrays were used to measure the changes in gene expression induced by 1,25D treatment of H37Rv-infected THP-1 cells

2013-11-28 | GSE52819 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data