Ontology highlight
ABSTRACT:
PROVIDER: PRJNA766812 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:<p>Energy metabolism is highly interdependent with adaptive cell migration <em>in vivo</em>. Mechanical confinement is a critical physical cue that induces switchable migration modes of the mesenchymal-to-amoeboid transition (MAT). However, the energy states in distinct migration modes, especially amoeboid-like stable bleb (A2) movement, remain unclear. In this report, we developed multivalent DNA framework-based nanomachines to explore strategical mitochondrial trafficking and differential ATP levels during cell migration in mechanically heterogeneous microenvironments. Through single-particle tracking and metabolomic analysis, we revealed that fast A2-moving cells driven by biomimetic confinement recruited back-end positioning of mitochondria for powering highly polarized cytoskeletal networks, preferentially adopting an energy-saving mode compared with a mesenchymal mode of cell migration. We present a versatile DNA nanotool for cellular energy exploration and highlight that adaptive energy strategies coordinately support switchable migration modes for facilitating efficient metastatic escape, offering a new perspective for therapeutic interventions in cancer metastasis.</p>
2024-03-25 | MTBLS9657 | MetaboLights
Project description:Collective cell migration is one of the principal modes for cancer cell movements. However, the triggering event for collective migration and its clinical significance is unclear. Here, we found that Snail, a major inducer of epithelial-mesenchymal transition (EMT), is critical for orchestrating collective migration in squamous cell carcinoma (SCC). To invstigate how Snail contribute to collective migration and invasion, we used microarrays to identify the global gene alterations regulated by Snail in SCC cells.
2018-08-25 | GSE87841 | GEO
Project description:We report expression profiles of various senescent cell types and modes of induction.
2024-04-01 | GSE230357 | GEO
Project description:We report expression profiles of various senescent cell types and modes of induction.
2023-11-02 | GSE230181 | GEO
Project description:We investigated two microenvironmental factors, tumor-intrinsic hypoxia, and tumor-secreted factors (secretome) as triggers of collective migration using a three-dimensional (3D) discrete-sized microtumor models that recapitulate hallmarks of Ductal carcinoma in situ (DCIS) to invasive ductal carcinoma (IDC) transition. These two factors induced two distinct modes of collective migration: directional and radial migration in the 3D microtumors generated from the same breast cancer cell line model, T47D. Without external stimulus, large (>500µm) T47D microtumors exhibited tumor-intrinsic hypoxia and directional collective migration while small (<150 µm), non-hypoxic microtumors exhibited radial collective migration only when exposed to secretome of large microtumors. To investigate the differences in the underlying mechanism present between hypoxia- and secretome-induced directional versus radial migration modes, we performed differential gene expression analysis of hypoxia- and secretome-induced migratory microtumors vs. non-hypoxic, non-migratory small microtumors as controls. We used microarrays to detail the global programme of gene expression profiling to study tumor intrinsic hypoxia induced directional migration and secretom induced radial migrartion in large 600µm microtumors with small 150μm microtumors as controls in three dimensional (3D) breast microtumor model
2021-03-26 | GSE166211 | GEO
Project description:Reiterer2013 - pseudophosphatase STYX role in
ERK signalling
This model is described in the article:
Pseudophosphatase STYX
modulates cell-fate decisions and cell migration by
spatiotemporal regulation of ERK1/2.
Reiterer V, Fey D, Kolch W,
Kholodenko BN, Farhan H.
Proc. Natl. Acad. Sci. U.S.A. 2013 Jul;
110(31): E2934-43
Abstract:
Serine/threonine/tyrosine-interacting protein (STYX) is a
catalytically inactive member of the dual-specificity
phosphatases (DUSPs) family. Whereas the role of DUSPs in
cellular signaling is well explored, the function of STYX is
still unknown. Here, we identify STYX as a spatial regulator of
ERK signaling. We used predictive-model simulation to test
several hypotheses for possible modes of STYX action. We show
that STYX localizes to the nucleus, competes with nuclear DUSP4
for binding to ERK, and acts as a nuclear anchor that regulates
ERK nuclear export. Depletion of STYX increases ERK activity in
both cytosol and nucleus. Importantly, depletion of STYX causes
an ERK-dependent fragmentation of the Golgi apparatus and
inhibits Golgi polarization and directional cell migration.
Finally, we show that overexpression of STYX reduces ERK1/2
activation, thereby blocking PC12 cell differentiation.
Overall, our results identify STYX as an important regulator of
ERK1/2 signaling critical for cell migration and PC12 cell
differentiation.
This model is hosted on
BioModels Database
and identified by:
BIOMD0000000557.
To cite BioModels Database, please use:
BioModels Database:
An enhanced, curated and annotated resource for published
quantitative kinetic models.
To the extent possible under law, all copyright and related or
neighbouring rights to this encoded model have been dedicated to
the public domain worldwide. Please refer to
CC0
Public Domain Dedication for more information.
2024-09-02 | BIOMD0000000557 | BioModels
Project description:Amoeboid and mesenchymal migration of cancer cells both contribute to metastatic spreading of tumors. To characterize gene expression profiles underlying the different migratory modes, we performed RNA sequencing of HT1080 fibrosarcoma cells undergoing mesenchymal-amoeboid transition induced by either doxycycline-inducible constitutively active RhoA or dasatinib treatment. Cells were kept in three-dimensional collagen gels with or without induction for 48 hours. RNA was isolated with a modified Chomczynski protocol. RNA-seq libraries were constructed from DNase I treated, rRNA depleted total RNA and sequenced with Illumina 2000/2500 sequencers.
2019-07-29 | E-MTAB-6823 | biostudies-arrayexpress
Project description:Cell migration is an essential process in health and disease - especially in cancer metastasis. The difficulty to assess migration in high throughput presents a methodological hurdle - hence, only very few screens revealed factors controlling this important process. Here, we introduce MigExpress as a platform for the "identification of Migration control genes by differential Expression". MigExpress exploits the combination of in-depth molecular profiling and the robust quantitative analysis of migration capacity in a broad panel of samples and identifies migration-associated genes by their differential expression in slowly versus fast migrating cells. We applied MigExpress to non-small cell lung cancer (NSCLC), the most frequent cause of cancer mortality mainly due to metastasis. In 54 NSCLC cell lines, we comprehensively determined mRNA and protein expression. Correlating the transcriptome and proteome profiles with the quantified migration properties led to the discovery and validation of FLNC, DSE, CPA4, TUBB6 and BICC1 as migration control factors in NSCLC cells, which also negatively correlated with patient survival. Notably, FLNC was the least expressed filamin in NSCLC, but the only one controlling cell migration and correlating with patient survival and metastatic disease stage.
2021-04-20 | PXD022146 | Pride