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RNAseq of two mouse LUSC cell lines derived from NTCU-induced model

ABSTRACT: RNAseq of two mouse LUSC cell lines derived from NTCU-induced model

PROVIDER: PRJNA767537 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR16122972.fastq.gz	Fastqsanger.gz
	SRR16122973.fastq.gz	Fastqsanger.gz
	SRR16122974.fastq.gz	Fastqsanger.gz
	SRR16122975.fastq.gz	Fastqsanger.gz
	SRR16122976.fastq.gz	Fastqsanger.gz

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RNAseq of two mouse LUSC cell lines derived from NTCU-induced model

Project description:We report RNAseq data form two independent mouse syngeneic LUSC cell lines UN-SCC679 and UN-SCC680 Carcinogenesis and cell line generation: LUSC tumors were induced by N-nitroso-tris-chloroethylurea (NTCU) (Toronto Research Chemicals) treatment applying 0.04 M NTCU by skin painting twice a week for 20 weeks to 8-week-old A/J mice. Lungs of euthanized mice were excised and tumor cell lines UN-SCC679 and UN-SCC680 were derived and cultured for 25 passes to ensure they were immortalized, subcutaneously injected into 6-week-old female Rag2-/-IL2Rg-/- immunodeficient mice flanks subcutaneously and finally engrafted in syngeneic 8-week-old A/J mice. These cell lines were cultured in RPMI 1640 supplemented with 10% Fetalclone (Thermo Fisher Scientific) and 100 U/mL penicillin-100 µg/mL streptomycin (Thermo Fisher Scientific). All cells were grown in a humidified incubator containing 5% CO2 at 37°C. Cell lines were routinely tested for mycoplasma.

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caArray_EXP-598: TCGA (LUSC): Analysis of DNA Methylation for LUSC using Illumina Infinium HumanMethylation450 platform (jhu-usc)

Project description:TCGA Analysis of DNA Methylation for LUSC using Illumina Infinium HumanMethylation450 platform

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caArray_EXP-598: TCGA (LUSC): Analysis of DNA Methylation for LUSC using Illumina Infinium HumanMethylation450 platform (jhu-usc)

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ChIP-Seq of BCL11A and SOX2 in lung squamous cell carcinoma (LUSC) LK-2 cell line with or without BCL11A knockdown

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Genome-wide maps of histone modifications in mouse tumor-derived cell lines

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Project description:The purpose of this study is to compare methylation profiles of LUSC between tumor tissue and normal tissue

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