Project description:We report here the genome-wide deposition of H3K9me3 and H3K27me3 in wild-type, Mfn2-/-, Vhl-/- and HIF1dPA regenerating myofibers.
Project description:Expression data from antigen-experienced Nfat1+/+ and Nfat1-/- CD4+ T cells following 21 days of Plasmodium yoelii 17XNL infection.
Project description:We isolated chondrocytes from an NFAT1 deficient patients osteochondromas surgically removed from the promixal tibia. The cells were either infected with empty vector (labeled "patient") or WT-NFATC2 (labeled "rescue"). We then conducted differential expression, and gene-set enrichment analysis and determined that inflammatory and cell proliferation signals were higher in "patient" chondrocytes
Project description:Spatial transcriptomics analysis of three different experiments (describe below) identify regenerating ACTC1+ myofibers as a supportive cell type for transplanted SMPCs. Data correspond to Figures 6-7 and Extended Data Figures 8-10 in Hicks et al., "Regenerating Human Skeletal Muscle Forms an Emerging Niche In Vivo to Support PAX7 cells".
Project description:We have generated CRISPR edited versions of hESC line MShef11 to produce MFN2 R94Q/+ and MFN2 R94Q/R94Q lines as a model for Charcot Marie Tooth Disease (CMT) 2A. This were differentiated to limb innervating motor neurons, the predominantly affected cell time in CMT2A and RNA was examined to investigate differences in cell lines.
Project description:MicroRNA-expression profile of dystrophic single fibres vs wild type single fibers isolated from different muscle of mdx and c57bl mice. Myofibers were isolated from different muscle type (tibialis, diaphragm and quadriceps of gender- (male) and age- (3 month old and half) matched wt and dystrophic mice). 9 total samples per animal model, 3 replicates per muscle type sample