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H3K9me3 and H3K27me3 ChIP-seq in wild-type, Mfn2-/-, Vhl-/- and HIF1dPA regenerating myofibers.

ABSTRACT: H3K9me3 and H3K27me3 ChIP-seq in wild-type, Mfn2-/-, Vhl-/- and HIF1dPA regenerating myofibers.

PROVIDER: PRJNA767660 | ENA |

REPOSITORIES: ENA

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H3K9me3 and H3K27me3 ChIP-seq in wild-type, Mfn2-/-, Vhl-/- and HIF1dPA regenerating myofibers.

Project description:We report here the genome-wide deposition of H3K9me3 and H3K27me3 in wild-type, Mfn2-/-, Vhl-/- and HIF1dPA regenerating myofibers.

2022-08-16 | GSE185103 | GEO

NFAT1 (NFATc2) ChIP-seq in wild-type and Mfn2-/- regenerating myofibers

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NFAT1 (NFATc2) ChIP-seq in wild-type and Mfn2-/- regenerating myofibers

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Spatial RNA sequencing analysis confirm ACTC1+ myofibers support a transplanted SMPC pool

Project description:Spatial transcriptomics analysis of three different experiments (describe below) identify regenerating ACTC1+ myofibers as a supportive cell type for transplanted SMPCs. Data correspond to Figures 6-7 and Extended Data Figures 8-10 in Hicks et al., "Regenerating Human Skeletal Muscle Forms an Emerging Niche In Vivo to Support PAX7 cells".

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Project description:We have generated CRISPR edited versions of hESC line MShef11 to produce MFN2 R94Q/+ and MFN2 R94Q/R94Q lines as a model for Charcot Marie Tooth Disease (CMT) 2A. This were differentiated to limb innervating motor neurons, the predominantly affected cell time in CMT2A and RNA was examined to investigate differences in cell lines.

2022-07-28 | E-MTAB-10831 | biostudies-arrayexpress

Dystrophic single myofibers vs control single myofibers

Project description:MicroRNA-expression profile of dystrophic single fibres vs wild type single fibers isolated from different muscle of mdx and c57bl mice. Myofibers were isolated from different muscle type (tibialis, diaphragm and quadriceps of gender- (male) and age- (3 month old and half) matched wt and dystrophic mice). 9 total samples per animal model, 3 replicates per muscle type sample

2012-12-21 | E-GEOD-21219 | biostudies-arrayexpress

Dystrophic single myofibers vs. control single myofibers

Project description:MicroRNA-expression profile of dystrophic single fibers compared to wild type single fibers isolated from different muscles of mdx and C57BL mice. Myofibers were isolated from different muscle types (tibialis, diaphragm and quadriceps) of gender- (male) and age- (3 month old and half) matched wt and dystrophic mice. 9 total samples per animal model (C57BL, mdx), 3 replicates per muscle type.

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Mouse regenerating myofibers detected as false-positive donor myofibers with anti-human spectrin.

Project description:Abstract Stem cell transplantation is being tested as a potential therapy for a number of diseases. Stem cells isolated directly from tissue specimens or generated via reprogramming of differentiated cells require rigorous testing for both safety and efficacy in preclinical models. The availability of mice with immune-deficient background that carry additional mutations in specific genes facilitates testing the efficacy of cell transplantation in disease models. The muscular dystrophies are a heterogeneous group of disorders, of which Duchenne muscular dystrophy is the most severe and common type. Cell-based therapy for muscular dystrophy has been under investigation for several decades, with a wide selection of cell types being studied, including tissue-specific stem cells and reprogrammed stem cells. Several immune-deficient mouse models of muscular dystrophy have been generated, in which human cells obtained from various sources are injected to assess their preclinical potential. After transplantation, the presence of engrafted human cells is detected via immunofluorescence staining, using antibodies that recognize human, but not mouse, proteins. Here we show that one antibody specific to human spectrin, which is commonly used to evaluate the efficacy of transplanted human cells in mouse muscle, detects myofibers in muscles of NOD/Rag1(null)mdx(5cv), NOD/LtSz-scid IL2Rγ(null) mice, or mdx nude mice, irrespective of whether they were injected with human cells. These "reactive" clusters are regenerating myofibers, which are normally present in dystrophic tissue and the spectrin antibody is likely recognizing utrophin, which contains spectrin-like repeats. Therefore, caution should be used in interpreting data based on detection of single human-specific proteins, and evaluation of human stem cell engraftment should be performed using multiple human-specific labeling strategies.

| S-EPMC3900004 | biostudies-literature

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