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Global genomic instability caused by reduced expression of DNA polymerase ε in yeast [DZP2-derived sectored colonies]

ABSTRACT: Global genomic instability caused by reduced expression of DNA polymerase ε in yeast [DZP2-derived sectored colonies]

PROVIDER: PRJNA768822 | ENA |

REPOSITORIES: ENA

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Global genomic instability caused by reduced expression of DNA polymerase ε in yeast [DZP2-derived sectored colonies]

Project description:Yeast Saccharomyces cerevisiae has been widely used as a model system for studying genome instability. Here, a heterozygous diploid S. cerevisiae strain DZP2 was generated to determine the genomic alterations induced by DNA polymerase ε. The expression of POL2 was regulated by the GAL1 promoter. In combination of a custom SNP microarray, the patterns of chromosomal instability induced by low Pol ε could be explored at a whole genome level in DZP2. Using this system, we found hundreds-fold higher rate of genomic alterations, including aneuploidy, loss of heterozygosity (LOH), and chromosomal rearrangement. DNA polymerase ε (Pol ε) is one of the three replicative eukaryotic DNA polymerases. Pol ε deficiency leads to genomic instability and multiple human diseases. Here, we explored global genomic alterations in yeast strains with reduced expression of POL2, the gene that encodes the catalytic subunit of Pol ε. Using whole-genome SNP microarray and sequencing, we found that low levels of Pol ε elevated the rates of mitotic recombination and chromosomal aneuploidy by two orders of magnitude. Strikingly, low levels of Pol ε resulted in a contraction of the number of repeats in the rDNA cluster and reduced the length of telomeres. These short telomeres led to an elevated frequency of break-induced replication, resulting in terminal loss of heterozygosity. In addition, low levels of Pol ε increased the rate of single-base mutations by 13-fold by a Pol ζ-dependent pathway. Finally, the patterns of genomic alterations caused by low levels of Pol ε were different from those observed in strains with low levels of the other replicative DNA polymerases Pol α and Pol δ, providing further insights into the different roles of the B-family DNA polymerases in maintaining genomic stability.

2021-10-07 | GSE185364 | GEO

Global genomic instability caused by reduced expression of DNA polymerase ε in yeast [DZP2-derived isolates]

2021-10-07 | GSE185365 | GEO

Global genomic instability caused by reduced expression of DNA polymerase ε in yeast

Project description:Global genomic instability caused by reduced expression of DNA polymerase ε in yeast

| PRJNA768812 | ENA

Global genomic instability caused by reduced expression of DNA polymerase ε in yeast [DZP2-derived isolates]

Project description:Global genomic instability caused by reduced expression of DNA polymerase ε in yeast [DZP2-derived isolates]

| PRJNA768823 | ENA

Genomic instability induced by furfural-treated in S. cerevisiae [sectored colonies]

Project description:Genomic instability induced by furfural-treated in S. cerevisiae [sectored colonies]

| PRJNA414089 | ENA

Global genomic instability caused by reduced expression of DNA polymerase ε in yeast

Project description:This SuperSeries is composed of the SubSeries listed below.

2021-10-07 | GSE185366 | GEO

colonies

Project description:colonies Metagenome

| PRJNA673756 | ENA

Karyotype analysis of radicicol-resistant yeast colonies by array CGH (Radr1,2,3)

Project description:Aneuploidy, a state of having uneven numbers of chromosomes, is a form of large-effect mutation able to confer adaptive phenotypes under diverse stress conditions. Here we investigate whether pleiotropic stresses could in turn induce aneuploidy in budding yeast. Cells exposed to radicicol, an Hsp90 inhibitor, exhibit chromosomal instability. To assess the karyotype of cells with an acquired resistance to radicicol, array CGH was used to compare three stably resistant yeast colonies to a WT strain. Single replicates of DNA prepared from radicicol-resistant yeast colonies are compared to a control WT colony.

2011-12-31 | E-GEOD-34085 | biostudies-arrayexpress

Genomic instability induced by furfural-treated in S. cerevisiae [sectored colonies]

Project description:Furfural is a key inhibitor in S. cerevisiae fermentation causing serious economic loss. To disclose of the recombinational DNA lesions induced by furfural, we analyzed 19 JSC25-derived sectored colonies using chromosome IV SNP-specific microarrays. The parent diploid S. cerevisiae strain JSC25 (constructed by crossing two haploid strains isogenic W303-1A and YJM789) was homologous for ade2-1 alleles. One copy of gene SUP4-o, an ochre-suppressing tRNA, was inserted at the end of right arm of chromosome IV. Under normal conditions, the colony color of JSC25 is pink. A crossover event occurred on the right arm of chromosome IV at the first cell cycle would result in a white/red sectored colony on solid plate.

2017-10-13 | GSE104880 | GEO

Karyotype analysis of radicicol-resistant yeast colonies by array CGH (Radr1,2,3)

2011-12-31 | GSE34085 | GEO