Project description:Human myofibroblastic LX2 cells were transfected using a siRNA directed against BNC2 (siBNC2) or a control siRNA (siCTRL) and RNA were processed for microarray analyses.
Project description:The goal of this study was to use Rapid Immunoprecipitation Mass spectrometry of Endogenous protein (RIME) assays (Mohammed et al., 2016) to identify transcriptional regulatory partners of the transcription factor BNC2 in LX2 cells. Protein complexes were immune-precipitated with an anti-BNC2 antibody (55220-1-AP, Proteintech) or control non-immune IgG (normal rabbit IgG #2729, Cell Signaling) before being subjected to MS.
Project description:Mouse myofibroblastic EMS404 cells were transfected using a siRNA directed against BNC2 (siBNC2) or a control siRNA (siCTRL) and RNA were processed for microarray analyses.