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Screening for eukaryotic motives in Legionella pneumophila reveals bacterial LPC_1677 as functional host histone de-acetylase

ABSTRACT: Screening for eukaryotic motives in Legionella pneumophila reveals bacterial LPC_1677 as functional host histone de-acetylase

PROVIDER: PRJNA771394 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR16345563.fastq.gz	Fastqsanger.gz
	SRR16345564.fastq.gz	Fastqsanger.gz
	SRR16345565.fastq.gz	Fastqsanger.gz
	SRR16345566.fastq.gz	Fastqsanger.gz
	SRR16345567.fastq.gz	Fastqsanger.gz

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Screening for eukaryotic motives in Legionella pneumophila reveals bacterial LPC_1677 as functional host histone de-acetylase

Project description:Purpose: The interaction between Legionella pneumophila and its host cell is not completly understood. The aim of this study was to identify eukaryotic-like bacterial factors which manipulate the host cell. Method: A stable overexpression of LPC_1677 was performed in THP-1 cells. The LPC_1677 overexpressing cells were infected with L.p. and RNA sequencing was performed. Key pro-inflammatory markers were validated via qPCR and ELISA. Results: Bioinformatic screening for eukaryotic-like motives in L.p. took us to LPC_1677 which deacetylates host histones and thereby attenuating gene transcription. Conclusion: This study demonstrated that the eukaryotic-like bacterial factor LPC_1677 de-acetylates Histones in the host cell.

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Translocated Legionella pneumophila small RNAs mimic eukaryotic miRNAs to dampen the host immune response

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