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Comparison of the expressed profiling between bulbs of virus-free and virus-accumulated garlic

ABSTRACT: Comparison of the expressed profiling between bulbs of virus-free and virus-accumulated garlic

PROVIDER: PRJNA771547 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR16352427_1.fastq.gz	Fastqsanger.gz
	SRR16352427_2.fastq.gz	Fastqsanger.gz
	SRR16352428_1.fastq.gz	Fastqsanger.gz
	SRR16352428_2.fastq.gz	Fastqsanger.gz
	SRR16352429_1.fastq.gz	Fastqsanger.gz

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Comparison of the expressed profiling between bulbs of virus-free and virus-accumulated garlic

Project description:To explore the influence of virus-accumulation on garlic growth, we produced a virus-free garlic using the landrace “Chalingzipisuan” with great accumulation of viruses, based on the shoot-tip culture. Then, using the viruses-accumulated garlic and corresponding virus-free garlic, we performed a transcriptomic investigation for the enlarging-growth bulbs, and identified 1,182 garlic genes with differential expression, suggesting these genes involved in the response to viruses-infection

2021-10-17 | GSE185962 | GEO

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Project description:To characterize the expression of 58219 genes annotated from garlic genome, the transcriptomes of seven tissues (including garlic sprouts, shoots, bulbs, flowers, roots, pesudostems, and leaves) were sequenced, and finally identified 45,750 genes expressed in various tissues investigated. In addition, the bulbs-transcriptomes in the eight stages of bulbs-development were sequenced, resulting in 6,234 genes isentified to show dynamic expression changes in the bulbs-developmental process.

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Project description:The garlic landrace of ‘Chalingzipisuan’ was used for transcriptome analysis. The axillary bud of garlic is at the base of clove, whereas the storage leaf at the upper clove provides essential nutrition for the germination and seedling growth. Therefore, the basal and storage clove were ere separately performed for RNA sequencing from the bulbs under three different developmental stages (i.e., enlarging growth, dormancy, and germination), generating in total 77-85 million reads.

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Project description:To explore the clove enlarging growth-related genes, we compared the transcriptome of bulbs whose cloves are before enlargement and under enlarging-growth, respectively, using three garlic accessions, Chalingzipisuan, Ershuizao, and Yuanjiangyangsuan. Consequently, 4,658 genes were identified with a differential expression in at least two of three examined accessions.

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Expression data from garlic-fed mouse

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transcriptome of storage and basal clove of garlic bulbs

Project description:transcriptome of storage and basal clove of garlic bulbs

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Transcription profiling of mouse C57BL/6J mice spleen samples after garlic feeding to investigate anti-atherosclerotic, anti-proliferative, hypolipidemic and chemopreventative effects of garlic

Project description:Garlic is a popular flavor enhancer in modern cuisines. Although anti-atherosclerotic, anti-proliferative, hypolipidemic and chemopreventative effects of garlic are known for a long time, the mechanisms of garlic as a dietary supplement ramain largely unknown. We used microarrays to analyze the global programme of gene expression in control (cellulose) and garlic-fed mice and identified erythropoietic and heme biosynthetic genes that could, in part, be responsible for the pleiotropic effects of garlic on health Experiment Overall Design: 8-week old male C57BL/6J mice were divided into two diet groups and maintained at an SPF facility. Mice were fed (ad libitum) a special diet (Yeh and Yeh, 1994) with 4% cellulose (Control group, 3 mice) or 4% lyophilized raw garlic powder (Treatment group, 3 mice). At the end of the 15-week treatment, spleen organs were used for RNA isolation and arraying.

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Expression data from livers of 15-weeks garlic-fed mice

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