Ontology highlight
ABSTRACT:
PROVIDER: PRJNA772592 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:A DNA barcode library for woody plants in tropical and subtropical China Targeted loci
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Project description:Methods for Quick DNA Barcode Reference Library Construction
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Project description:Temperature is an important factor that affects the growth and reproduction of mangrove plants. Laguncularia racemosa (L.) C.F. Gaertn is a controversial species in China, in terms of being a pioneer species for mangrove restoration and a putative invasive species occupying the natural habitats. One of its advantages is the strong resistance to low temperature, which makes L. racemose more adaptive to extreme climate change than local mangrove species. However, little is known about the regulatory mechanisms underlying the cold-stress tolerance in L. racemose, which restricted our understanding on its biological features and invasion potential. In this study, In the current study, we investigated the physiological and molecular mechanisms involved in chilling-stress adaptation in L. racemose. Freezing temperature caused damage to cell membrane system and reduced photosynthesis efficiency in L. racemose. To combat the adverse impacts, plasma membrane biosynthesis and antioxidant processes were substantially enhanced. Furthermore, we showed that there was a difference between the responses to freeze-thaw injury in L. racemose from different locations. It may provide new clues to the different genetic background between varieties of L. racemose. These novel findings could provide biochemical and genetic basis for the cultivation and restoration of L. racemose.
2023-05-09 | GSE208631 | GEO
Project description:To identify key genes in the regulation of salt tolerance in the mangrove plant Bruguiera gymnorhiza, the transcriptome profiling under salt stress was carried out. Main roots and lateral roots were collected from the mangrove plants at 0, 1, 3, 6, 12 and 24 h, 3 6 and 12 days after NaCl-treatment. Keywords: time course, stress response, root type comparison
2008-05-01 | GSE10942 | GEO
Project description:HCC827 cells were barcoded using the ClonTracer lentiviral barcode library such that the majority of cells were infected with a single barcode. One million cells were expanded to ~120 million cells and split into 8 HYPERfasks. Two HYPERfasks were grown under DMSO and grown until confluence. In six HYPERfasks cells were grown under a GI90 concentration of one of two different inhibitors, gefitinib and trametinib (3 HYPERfasks each). Cells achieved confluence at 4 and 9 weeks for gefitinib and trametinib respectively. During this time, the medium and inhibitor were replenished weekly and DNA was extracted from the medium to track barcode content from dying cells.
2020-08-18 | E-MTAB-8841 | biostudies-arrayexpress
Project description:Sequencing of a random DNA barcode library containing ~491,587 barcodes
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Project description:Organoids were generated from cells infected with pooled lentivirus library. The lentiviral libraries deliver dual-gRNAs targeting 36 high-risk ASD genes in parallel. With or without 4-OHT induction of eCas9 expression, organoids were dissociated at specific developmental stages. Single cells were sorted and bulk genomic DNA was extracted. Target amplification was performed using genomic DNA to analyze gRNA and unique clone barcode representation.
2023-07-09 | E-MTAB-13140 | biostudies-arrayexpress
Project description:To identify key genes in the regulation of salt tolerance in the mangrove plant Bruguiera gymnorhiza, the transcriptome profiling under salt stress was carried out. Main roots and lateral roots were collected from the mangrove plants at 0, 1, 3, 6, 12 and 24 h, 3 6 and 12 days after NaCl-treatment. Samples were collected from each set of three trees at 0, 1, 3, 6, 12 and 24 h, 3 6 and 12 days after NaCl-treatment, that is 27 trees were used in total. RNA was extracted from mixture of the samples from 3 trees to average genotypes of 3 trees.
2008-05-01 | E-GEOD-10942 | biostudies-arrayexpress
Project description:Mangrove plants are the demonstrated woody plants in tropical and subtropical coastal area with great ecological and economic importance. However, due to the extreme global climate change and increased human activities, mangroves is suffering a dramatic declining worldwide. Lumnitzera littorea (Jack) Voigt is one of the most endangered mangrove species in China. A comprehensive understanding on its resistance mechanisms to low ambient temperature help us to better conserve and restore L. littorea. In the current study, we performed comparative transcriptome analysis to investigate the genome-wide changes of gene expression profile in L. littorea under chilling stress (8°C day/5°C night) over normal condition (25°C day/23°C night). The low temperature suppressed fatty acid elongation and protein phosphorylation in L. littorea, while induced calcium ion binding process and signaling transduction, suggesting an activation of cold-stress sensing and signaling in L. littorea. Combining our analysis with our previous physiological assays, we showed a substantial photoinhibition occurring in the seedlings of L. littorea with the decrease of ambient temperature, and the synthesis of photosystem II reaction center protein D1 and peroxidase-involved scavenging of reactive oxygen species (ROS) were enhanced accordingly to combat the adverse impacts. Finally, we highlighted the biological significance of post-transcriptional regulation, including RNA binding and surveillance, in coping with cold stress in L. littorea. Collectively, our findings provide a global view to the resistance mechanisms of chilling stress in L. littorea, and valuable genetic resources to assist the protection and restoration of L. littorea.
2023-09-27 | GSE211695 | GEO