Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Single-cell RNA-seq analysis of macrophages extracted from mouse alveolar bone subjected to orthodontic force

ABSTRACT: Single-cell RNA-seq analysis of macrophages extracted from mouse alveolar bone subjected to orthodontic force

PROVIDER: PRJNA772791 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Single-cell RNA-seq analysis of macrophages extracted from mouse alveolar bone subjected to orthodontic force

Project description:Exploring the transcriptome heterogeneity of macrophages during orthodontic force-induced alveolar bone remodeling

2022-02-01 | GSE186185 | GEO

Transcriptional Expression in Human Periodontal Ligament Cells Subjected to Orthodontic Force: An RNA-Sequencing Study

Project description:Purpose : This study was performed to investigate the changes in gene expression in periodontal ligament (PDL) cells following mechanical stimulus through RNA sequencing. Method : Premolars extracted for orthodontic treatment were used. To stimulate the PDL cells, an orthodontic force of 100× g was applied to the premolar (experimental group; n = 11), whereas the tooth on the other side was left untreated (control group; n = 11). After the PDL cells were isolated from the extracted teeth, gene set enrichment analysis (GSEA), differentially expressed gene (DEG) analysis, and real-time PCR were performed to compare the two groups. Result : GSEA demonstrated that gene sets related to the cell cycle pathway were upregulated in PDL. Thirteen upregulated and twenty downregulated genes were found through DEG analysis. Real-time PCR results confirmed that five upregulated genes (CC2D1B, CPNE3, OPHN1, TANGO2, and UAP-1) and six downregulated genes (MYOM2, PPM1F, PCDP1, ATP2A1, GPR171, and RP1-34H18.1-1) were consistent with RNA sequencing results. Conclusion : Two upregulated genes, CPNE3 and OPHN1, and one downregulated gene, PPM1F, play an important role in PDL regeneration in humans when orthodontic force is applied.

2021-02-03 | GSE165988 | GEO

RNA-seq analysis of RNA extracted from control alveolar bone samples and alveolar bone under apical periodontitis

Project description:To investigate the underlying mechanisms in the the states of apical periodontitis, we extracted RNA from control alveolar bone and alveolar bone under apical periodontitis.

2023-02-11 | GSE221990 | GEO

Transcriptional Expression in Human Periodontal Ligament Cells Subjected to Orthodontic Force: An RNA-Sequencing Study

Project description:Transcriptional Expression in Human Periodontal Ligament Cells Subjected to Orthodontic Force: An RNA-Sequencing Study

| PRJNA698902 | ENA

The long non-coding RNA landscape of periodontal ligament stem cells subjected to compressive force

Project description:To explore the lncRNA landscape of periodontal ligament stem cells (PDLSCs) subjected to compressive force.

2021-07-09 | GSE116787 | GEO

RNA-seq analysis of RNA extracted from control alveolar bone samples and alveolar bone under apical periodontitis

Project description:RNA-seq analysis of RNA extracted from control alveolar bone samples and alveolar bone under apical periodontitis

| PRJNA917270 | ENA

Proteomic analysis of human alveolar bone

Project description:We optimised a sample preparation and analysis workflow for proteomic analysis of human alveolar bone. We compared the peptides and proteins extracted from bone by different buffers, then used a multi-enzyme, multi-search engine approach to increase proteome coverage.

2019-11-27 | PXD011524 | Pride

The role of Type VI collagen in alveolar bone

Project description:In this study, an α2(VI) deficient mouse (Col6α2-KO) model was used to examine the role of Type VI collagen in oral tissues. To examine bone properties, µCT was employed, and bone volume and bone mineral density (BMD) was measured in oral tissues. To further investigate its molecular basis, proteome analysis was performed using protein extracted from alveolar bone. In addition, alveolar bone loss progression was evaluated with a periodontitis induced model. µCT analysis showed the Col6α2- KO mice had less volume of alveolar bone, dentin and dental pulp, while the width of periodontal ligament (PDL) was greater than WT. The BMD in alveolar bone and dentin were elevated in Col6α2-KO mice compared with WT. Our proteome analysis showed significant changes in proteins related to ECM organization and elevation of proteins associated with biomineralization in the Col6α2-KO mice. In induced periodontitis, Col6α2-KO mice had greater alveolar bone loss compared to WT. In conclusion, Type VI collagen has role in controlling biomineralization in alveolar bone and that changes in the ECM of alveolar bone could be associated with greater bone loss from periodontitis.

2022-11-21 | PXD037422 | Pride

Differential gene expression of static and intermittent compressive force treated human periodontal ligament cells

Project description:Mechanical force has been shown to regulate periodontal ligament cells (PDLs) behaviors. However, different force types lead to distinct PDLs’ responses. Here, the differential gene expression profiling of PDLs subjected to static and intermittent compressive forces was examined using RNA sequencing technique. Results demonstrated that the static and intermittent compressive force treated PDLs exhibited the differential regulation genes. KEGG pathway enrichment analysis revealed that focal adhesion and transforming growth factor beta signaling pathway were commonly upregulated while calcium signaling pathway was downregulated in both static and intermittent compressive force-treated PDLs. Interestingly, Wnt signaling pathway was upregulated only in the PDLs that subjected to the intermittent compressive force.

2019-07-23 | GSE112122 | GEO

The long non-coding RNA landscape of periodontal ligament stem cells subjected to compressive force

Project description:The long non-coding RNA landscape of periodontal ligament stem cells subjected to compressive force

| PRJNA480245 | ENA