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The trans-acting long noncoding RNA FLAIL represses flowering in Arabidopsis.

ABSTRACT: The trans-acting long noncoding RNA FLAIL represses flowering in Arabidopsis.

PROVIDER: PRJNA772961 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR16497716_1.fastq.gz	Fastqsanger.gz
	SRR16497716_2.fastq.gz	Fastqsanger.gz
	SRR16497717_1.fastq.gz	Fastqsanger.gz
	SRR16497717_2.fastq.gz	Fastqsanger.gz
	SRR16497718_1.fastq.gz	Fastqsanger.gz

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The trans-acting long noncoding RNA FLAIL represses flowering in Arabidopsis [RNA-seq]

Project description:The trans-acting long noncoding RNA FLAIL represses flowering in Arabidopsis [RNA-seq]

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The trans-acting long noncoding RNA FLAIL represses flowering in Arabidopsis [ChIRP-seq]

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The trans-acting long noncoding RNA FLAIL represses flowering in Arabidopsis [ChIRP-seq]

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The trans-acting long noncoding RNA FLAIL represses flowering in Arabidopsis.

Project description:This SuperSeries is composed of the SubSeries listed below.

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The long noncoding RNA Pnky is a trans-acting regulator of cortical development in vivo

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The long noncoding RNA Pnky is a trans-acting regulator of cortical development in vivo

Project description: While it is now appreciated that certain long noncoding in vivo, particularly with genetic strategies that establish cis versus trans mechanisms. Pnky is a nuclear-enriched lncRNA that is transcribed divergently from the neighboring proneural transcription factor Pou3f2. Here we show that conditional deletion of Pnky from the developing cortex regulates the production of projection neurons from neural stem cells (NSCs) in a cell-autonomous manner, altering postnatal cortical lamination. Surprisingly, Pou3f2 expression is not disrupted by deletion of the entire Pnky gene. Moreover, expression of Pnky from a BAC transgene rescues the differential gene expression and increased neurogenesis of Pnky-knockout NSCs, as well as the developmental phenotypes of Pnky-deletion in vivo. Thus, despite being transcribed divergently from a key developmental transcription factor, the lncRNA Pnky regulates development in trans.

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Somatic Primary piRNA Biogenesis Driven by Cis-Acting RNA Elements and Trans- Acting Yb

Project description:Primary piRNAs in Drosophila ovarian somatic cells arise from piRNA cluster transcripts and the 3′ UTRs of a subset of mRNAs, including Traffic jam (Tj) mRNA. However, it is unclear how these RNAs are determined as primary piRNA sources. Here, we identify a cis-acting 100-nt fragment in the Tj 3′ UTR that is sufficient for producing artificial piRNAs from unintegrated DNA. These artificial piRNAs were effective in endogenous gene transcriptional silencing. Yb, a core component of primary piRNA biogenesis center Yb bodies, directly bound the Tj-cis-element. Disruption of this interaction markedly reduced piRNA production. Thus, Yb is the trans-acting partner of the Tj-cis-element. Yb-CLIP revealed that Yb-binding correlated with somatic piRNA production but Tj-cis-element downstream sequences produced few artificial piRNAs. Thus, Yb determines primary piRNA sources by two modes of action; primary binding to cis-elements to specify substrates, and secondary binding to downstream regions to increase diversity in piRNA populations. HITS-CLIP of Yb in OSCs (Ovarian Somatic Cells) depleted for tj cis-element, and small RNA sequencing of Piwi-piRNAs in OSCs depleted for tj cis-element.

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