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Identification of Aurora B pathway involved in the anti-cancer effects by Paeoniae Radix extracts based on whole transcriptome analysis

ABSTRACT: Identification of Aurora B pathway involved in the anti-cancer effects by Paeoniae Radix extracts based on whole transcriptome analysis

PROVIDER: PRJNA773034 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR16499200.fastq.gz	Fastqsanger.gz
	SRR16499201.fastq.gz	Fastqsanger.gz
	SRR16499202.fastq.gz	Fastqsanger.gz
	SRR16499203.fastq.gz	Fastqsanger.gz
	SRR16499204.fastq.gz	Fastqsanger.gz

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The analysis of gene transcription and the effect of drug resistance of Radix Paeoniae Rubra Decoction on Staphylococcus aureus

Project description:To study the effect of Radix Paeoniae Rubra decoction on tolerance of Staphylococcus aureus.The effect of Radix Paeoniae Rubra on the resistance of Staphylococcus aureus to oxacillin sodium was studied by millipore dilution method in this experiment.At the same time ,conducted on transcriptome analysis of Staphylococcus aureus related genes in Radix Paeoniae Rubra.And to detect the expression level of related genes of Staphylococcus aureus under the action of Radix Paeoniae Rubra by PCR technology.The tolerance of Staphylococcus aureus was decreased obviously when the concentration of Radix Paeoniae Rubra decoction was above 1mg/ml.The effect of Radix Paeoniae Rubra decoction on the expression of tolerance genes femB,pvL and gluM when the concentration of Radix Paeoniae Rubra decoction was above 4mg/ml.When rhe concentration of Radix Paeoniae Rubra is more than 1mg/ml,it can effectively reduce the resistance of Staphylococcus aureus to oxacillin sodium.The reason may be due to the effect of Radix Paeoniae Rubra on the resistance gene of Staphylococcus aureus.

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Project description:Epidermal growth factor receptor (EGFR) inhibitors, as targeted therapies for non-small-cell lung cancer (NSCLC), have significantly enhanced patient survival and quality of life. However, despite these advancements, a significant proportion of patients exhibit resistance to EGFR inhibitors, limiting their overall treatment effectiveness. This study investigates the synergistic effects of combining Paeoniae Radix (PR) with the EGFR inhibitors erlotinib and gefitinib to overcome this resistance. The transcriptomic analysis of PR treatment revealed its potential to reverse the gene signature associated with resistance to EGFR inhibitors, as identified through analysis of a cell line database in EGFR mutant NSCLC. Combination treatment experiments validated that PR increased responsiveness to erlotinib and gefitinib in H1650 and H1975 NSCLC cells. By combining molecular experiments and transcriptome analysis, we found that PR may suppress resistance by modulating the Aurora B and apoptosis pathways. Notably, the combination therapy upregulated the apoptosis pathway and downregulated the Aurora B pathway more than single drug treatments. These results may contribute to the development of natural product-based combination therapeutic strategies to inhibit drug resistance in NSCLC.

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Identification of Aurora B pathway involved in the anti-cancer effects by Paeoniae Radix extracts based on whole transcriptome analysis

Project description:we profiled the change in genome-wide gene expression of human lung cancer cells in response to treatment of water and ethanol extracts of PR and its ingredients in a dose dependent manner to investigate PR efficacy at the molecular pathway level.

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