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Identification of Aurora B pathway involved in the anti-cancer effects by Paeoniae Radix extracts based on whole transcriptome analysis

ABSTRACT: we profiled the change in genome-wide gene expression of human lung cancer cells in response to treatment of water and ethanol extracts of PR and its ingredients in a dose dependent manner to investigate PR efficacy at the molecular pathway level.

ORGANISM(S): Homo sapiens

PROVIDER: GSE186218 | GEO | 2022/12/31

REPOSITORIES: GEO

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Identification of Aurora B pathway involved in the anti-cancer effects by Paeoniae Radix extracts based on whole transcriptome analysis

Project description:Identification of Aurora B pathway involved in the anti-cancer effects by Paeoniae Radix extracts based on whole transcriptome analysis

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High-throughput transcriptomics of water extracts detects reductions in biological activity with water treatment processes

Project description:The presence of numerous chemical contaminants from industrial, agricultural, and pharmaceutical sources in water supplies poses a potential risk to human and ecological health. Current chemical analyses suffer from limitations including chemical coverage and high cost, and broad-coverage in vitro assays such as transcriptomics may further improve water quality monitoring by assessing a large range of possible effects. Here, we used high-throughput transcriptomics to assess the activity induced by field-derived water extracts in MCF7 breast carcinoma cells.

2024-03-05 | GSE252117 | GEO

Identification of the pharmacological effects of Chrysanthemum morifolium Ramat. leaves extracts on high-fat diet induced obese mice through lipid-, and glucose metabolism

Project description:We investigated that ethanol extracts of Chrysanthemum morifolium Ramat. leaves improved metabolic disease on high-fat diet induced obese mice using metabolic profiling of lipid-, glucose, inflammation and RNA-seq transcriptomic profling.

2019-04-24 | GSE130218 | GEO

1H NMR spectroscopy-based metabolic profiling of Ophiocordyceps sinensis and Cordyceps militaris of water-boiled and 50% ethanol-soaked extracts

Project description:Introduction Ophiocordyceps sinensis, a well-known Chinese complementary herb, is a rare and valuable therapeutic resource. Cordyceps militaris (C. militaris) is a commonly used substitute for O. sinensis. A metabolomic-based approach for exploring the similarities and differences in the metabolites of O. sinensis and C. militaris in water-boiled and 50% ethanol-soaked extracts is of great significance. Objectives To determine a vital role of extraction methodologies in influencing the metabolic composition of herbs, 1HNMR-based profiling was used to characterize the metabolic fingerprints of O. sinensis and C. militaris. Methods To make a distinction between the global metabolite profiling of O. sinensis and C. militaris extracts obtained from either the water-boiled or 50% ethanol-soaked methods, we screened the herbs samples using 1HNMR-based metabolic fingerprints combined with multivariate statistical analysis. Results This study revealed that a total of 43 (82.69% of 52) metabolites were detectable in both O. sinensis and C. militaris. According to the variable importance in projection (VIP) value and p-value from the Mann-Whitney test, 7 metabolites (alanine, aspartate, glutamate, mannitol, ornithine, serine, and trehalose) differed between O. sinensis and C. militaris. Arginine, glucose, putrescine, pyroglutamate, betaine, O-phosphocholine, and xylose differed significantly between the water-boiled and 50% ethanol-soaked methods used to prepare the herb extracts. Conclusion A total of 52 primary metabolites were identified and quantified from O. sinensis and C. militaris samples. The study suggests that a water-boiled extraction is much faster method and strongly recommended over the 50% ethanol-soaked method for both O. sinensis and C. militaris.

2019-04-28 | ST001189 | MetabolomicsWorkbench

Gene expression profiles of human mesenchymal stem cells exposed to surface water organic extracts

Project description:To assess the impact of surface water across the Hun River, several sampling sites located in the mainstream and the tributary were selected representative of pollution gradient and different pollution source. Human mesenchymal stem cells were exposed to organic extracts of surface water from six sites for 2 days. Microarrays were used to measure the gene expression. And the gene expression profiles were used to evaluate the ability of determine the potential biological effects, to differentiate different pollution source, and to identify the toxic components.

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the anti lipid accumulation and anti liver cancer formation effect of CACF in CD36 overexpression transgenic zebrafish

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The Study of Gene Expression Profiles of KG-1 Cells Treated with Antrodia Camphorata Extracts using cDNA microarray.

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Proteomics analysis of the effects of Grammatophyllum speciosum extracts on RAW 264.7 cells

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