Quantitative profiling of differentially expressed miRNAs in siCTL- and siOASL-transfected HUVECs
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ABSTRACT:
PROVIDER: PRJNA773487 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR16525306.fastq.gz | Fastqsanger.gz | |||
| SRR16525307.fastq.gz | Fastqsanger.gz | |||
| SRR16525308.fastq.gz | Fastqsanger.gz | |||
| SRR16525309.fastq.gz | Fastqsanger.gz | |||
| SRR19546547.fastq.gz | Fastqsanger.gz |
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Project description:Purpose: The goal of this study is to compare endothelial transcriptome affected by OASL knockdown under basal or stimulated conditions by utilizing RNA-seq. Methods: Endothelial mRNA profilies of siCTL or siOASL transfected HUVECs were generated by illumina sequencing method, in duplicate. Following an optimized data analysis workflow, RNA sequence reads were counted using StringTie-2.1.3b and used to identify genes differentially expressed between siCTL- and siOASL-transfected HUVECs treated with or without TNFα and IFNγ. Results: DESeq2 was used to identify genes differentially expressed between siCTL- and siOASL-transfected HUVECs under basal or stimulated conditions. Genes showing <10 read counts total were pre-filtered. Conclusions: Our study represents the first analysis of endothelial mRNA affected by OASL knockdown with biologic replicates, generated by RNA-seq.
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