Project description:To further development of our gene expression approch to intracellular pathogenic bacterial controlling, we have employed castamized Neochlamydia S13 genomic microarray as a discovery platform to identy genes with the potential to inhibit Legionella growth into hoat amoebae , based on our data that the the amoebae haboring amoebal endosymbiont Neochlamydia S13 (an environmental chlamydia) could evade Legionella infection.
Project description:We performed shallow whole genome sequencing (WGS) on circulating free (cf)DNA extracted from plasma or cerebrospinal fluid (CSF), and shallow WGS on the tissue DNA extracted from the biopsy in order to evaluate the correlation between the two biomaterials. After library construction and sequencing (Hiseq3000 or Ion Proton), copy number variations were called with WisecondorX.
