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Histone variant H3.3 facilitates open chromatin at promoters [GRO-seq]

ABSTRACT: Histone variant H3.3 facilitates open chromatin at promoters [GRO-seq]

PROVIDER: PRJNA775070 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR16597137.fastq.gz	Fastqsanger.gz
	SRR16597138.fastq.gz	Fastqsanger.gz
	SRR16597139.fastq.gz	Fastqsanger.gz
	SRR16597140.fastq.gz	Fastqsanger.gz

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Histone variant H3.3 facilitates open chromatin at promoters.

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Histone variant H3.3 facilitates open chromatin at promoters [ChIP-seq]

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Histone variant H3.3 facilitates open chromatin at promoters [GRO-seq]

Project description:We analyze the contribution of H3.3 to chromatin accessibility, post-translational modification states, and transcription in mouse embryonic stem cells (mESC).

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Genome-wide incorporation dynamics reveal distinct categories of turnover for the histone variant H3.3

Project description:We developed a system to study the DNA replication-independent turnover nucleosomes containing the histone variant H3.3 in mammalian cells. By measuring the genome-wide incorporation of H3.3 at different time points following epitope-tagged H3.3 expression, we find three categories of H3.3-nucleosome turnover in vivo: rapid turnover, intermediate turnover and, specifically at telomeres, slow turnover. Our data indicate that H3.3-containing nucleosomes at enhancers and promoters undergo a rapid turnover that is associated with active histone modification marks including H3K4me1, H3K4me3, H3K9ac, H3K27ac and the histone variant H2A.Z. The rate of turnover is negatively correlated with H3K27me3 at regulatory regions and with H3K36me3 at gene bodies. Examination of incorporation dynamics of histone variant H3.3

2013-10-23 | E-GEOD-51505 | biostudies-arrayexpress

Histone variant H3.3 facilitates open chromatin at promoters [ChIP-seq]

Project description:We analyze the contribution of H3.3 to chromatin accessibility, post-translational modification states, and transcription in mouse embryonic stem cells (mESC).

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Histone variant H3.3 facilitates open chromatin at promoters [ATAC-seq]

Project description:We analyze the contribution of H3.3 to chromatin accessibility, post-translational modification states, and transcription in mouse embryonic stem cells (mESC).

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Histone H1 binding is restricted by histone variant H3.3

Project description:This SuperSeries is composed of the following subset Series: GSE16882: Histone H1 binding is restricted by histone variant H3.3 (Nucleosome) GSE16883: Histone H1 binding is restricted by histone variant H3.3 (DamID) GSE16884: Histone H1 binding is restricted by histone variant H3.3 (Expression) GSE19764: Histone H1 binding is restricted by histone variant H3.3 (FAIRE) Refer to individual Series

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