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ABSTRACT: Panax vietnamensis varieties sequencing

PROVIDER: PRJNA775868 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR16681232_1.fastq.gz	Fastqsanger.gz
	SRR16681232_2.fastq.gz	Fastqsanger.gz

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Panax vietnamensis

Project description:Panax vietnamensis overview

| PRJNA391367 | ENA

Transcriptome analysis of 1-year-old Panax vietnamensis Ha et Grushv.

Project description:Purpose: Panax vietnamensis Ha et Grushv., which contains various valuable ginsenosides, is an important herbal medicine of Vietnam. However, it is an endangered species listed in Vietnam Red Data Book due to over-harvseting. Investigation about genomic or trancriptomic resources is one of the necessary activities to conserve P. vietnamensis Materials and Methods:. In this study, we sequenced the transcriptomes of 1-year-old P. vietnamensis from leaves and roots using Illumina NovaSeqTM6000 system. Results: A total of 60,254,062 and 64,588,528 reads was obtained and then assembled into 45,495 and 49,133 unigenes for leaves and roots, respectively. More than 60% unigenes from two organs were functional annotated using at least one database among Kyoto Encyclopedia of Genes and Genomes, Pfam, Gene Ontology, NCBI non-redundant Protein, and Evolutionary genealogy of genes. Further, the predominant transcripts of each cDNA library were analyzed for different gene expression identification. In addition, 457 unigenes encoding enzymes involved in triterpenoid saponin biosynthesis via the mavelonate (MVA) and the non-MVA (also named as MEP pathways) were discovered.

2021-10-09 | GSE171608 | GEO

Suspension Cell Culture of Panax vietnamensis as a biotechnological source of ginsenosides: Growth, Cytology, and Ginsenoside Profile Assessment

Project description:Raw data of UPLC-qOrbitrap profiling results of extract from Cell Culture of Panax vietnamensis and standart compounds mixtures. In case of extract profiling, target list based DDA was conducted based on previous peak picking results.

2023-12-07 | MSV000093597 | MassIVE

Panax vietnamensis

Project description:Whole genome sequencing project of Panax vietnamensis Ha et Grushv.

| PRJNA665343 | ENA

Effect of Panax Notoginseng on gene expression in rats with cerebral ischemia-reperfusion injury

Project description:Methods: Panax notoginseng was used to treat MCAO model rats, and the differentially expressed genes between Panax notoginseng group and model group were identified by RNA SEQ, and the possible mechanism of Panax notoginseng in regulating ischemic stroke was analyzed

2020-09-13 | GSE154098 | GEO

GNPS Massive - Panax species adventitious roots (MSe / Negative ion mode)

Project description:A LC-MS/MS (Data-independent acquisition in negative ion mode) dataset from adventitious root cultures of four Panax species (P. ginseng, P. quinquifolius, P. vietnamensis, and P. notoginseng.

2021-08-10 | MSV000087966 | GNPS

Transcriptome analysis of 1-year-old Panax vietnamensis Ha et Grushv.

Project description:Transcriptome analysis of 1-year-old Panax vietnamensis Ha et Grushv.

| PRJNA720227 | ENA

Project description:Target capture reveals the complex origin of Vietnamese ginseng (Panax vietnamensis)

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Transcriptional profiling by high throughput sequencing of Panax ginseng C.A. Meyer leaf and stem transcriptomes during the leaf-expansion period.

Project description:In order to research the ginseng leaf-stem gene expression profiles of and dig out its function genes in the leaf-expansion period, the transcriptomic sequencing technology was set up the first time for five years the transcription of the Panax ginseng leaf-stem in the leaf-expansion period.

2012-07-31 | E-MTAB-937 | biostudies-arrayexpress

Nitrate fertilizer alleviates ammonium toxicity by enhancing ACLA-3 in Panax notoginseng roots

Project description:Nitrogen is one of the essential elements for plant growth. NH4+ and NO3- are two major forms of absorbing element N for higher plants. In this study we found that the growth of Panax notoginseng is inhibited when only adding ammonium nitrogen fertilizer, and adding nitrate fertilizer can alleviate the toxicity caused by ammonium. We use RNA-seq to identify genes that are related to the alleviated phenotypes after introducing NO3- to Panax notoginseng roots under NH4+ stresses. Twelve RNA-seq profiles in four sample groups, i.e., control, samples treated with NH4+, samples treated with NO3- only, and treated with both NH4+ and NO3- were obtained and analyzed to identify deregulated genes in samples with different treatments. ACLA-3 gene is downregulated in NH4+ treated samples, but is upregulated in samples treated with NO3- and with both NH4+ and NO3-, which is further validated in another set of samples using qRT-PCR. Our results suggest that unbalanced metabolism of nitrogen and nitrogen is the main cause of ammonium poisoning in roots of Panax notoginseng, and NO3- may significantly upregulate the activity of ACLA-3 which subsequently enhances the citrate cycle and many other metabolic pathways in Panax notoginseng root. These potentially increase the integrity of the Panax notoginseng roots. Our results suggest that introducing NO3- fertilizer is an effective means to prevent the occurrence of toxic ammonium in Panax notoginseng root.

2019-12-31 | GSE112437 | GEO

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