Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Characterisation of iPSC derived Sensory neurons with Single Cell RNA-seq

ABSTRACT: Characterisation of iPSC derived Sensory neurons with Single Cell RNA-seq

PROVIDER: PRJNA778177 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR16816465_1.fastq.gz	Fastqsanger.gz
	SRR16816465_2.fastq.gz	Fastqsanger.gz
	SRR16816466_1.fastq.gz	Fastqsanger.gz
	SRR16816466_2.fastq.gz	Fastqsanger.gz
	SRR16816467_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 6

Similar Datasets

Characterisation of iPSC derived Sensory neurons with Single Cell RNA-seq

Project description:Despite the rapid development of protocols to differentiate human pluripotent stem cells (hPSCs) into neurons, those used to produce sensory neurons remain difficult to replicate and result in heterogenous populations. Moreover, there is a growing clinical burden of chronic pain conditions, highlighting a need for relevant human cellular models. This study presents a hybrid differentiation method to produce nociceptive sensory neurons from hPSCs. Cell lines harbouring an inducible NEUROG2 construct were patterned towards precursors with small molecules, followed by NEUROG2 overexpression. We profilled cells with single cell RNA sequencing, which revealed populations of nociceptors, demonstrated by the expression of key developmental and fucntional genes including volatge gated sodium and TRP channels. Overall, this study represents an optimisation of existing methods to efficiently produce nociceptive sensory neurons and provides a tool for chronic pain research and the development of treatments.

2022-11-17 | GSE188272 | GEO

Effect of SARS-CoV-2 infection on gene expression of iPSC derived sensory neurons [scRNAseq]

2023-08-04 | GSE227529 | GEO

Gene expression from human iPSC derived motor neurons.

Project description:Gene expression from human iPSC derived motor neurons.

| EGAS00001003785 | EGA

FGF21 treatment and iPSC-derived neurons

Project description:FGF21 treatment and iPSC-derived neurons

| PRJNA925172 | ENA

Effect of SARS-CoV-2 infection on gene expression of iPSC derived sensory neurons

Project description:Effect of SARS-CoV-2 infection on gene expression of iPSC derived sensory neurons

| PRJNA945456 | ENA

Effect of SARS-CoV-2 infection on gene expression of iPSC derived sensory neurons [bulkRNAseq]

Project description:To investigate the impact of SARS-CoV-2 on the peripheral nervous system, we generated human sensory neurons from induced pluripotent stem cells (iPSC) that we further infected with the SARS-CoV-2 strain WA1/2020 for 48 hours. We then performed gene expression profiling analysis using data obtained from RNA-seq of mock treated cells compared to SARS-CoV-2 infected cells.

2023-08-04 | GSE227528 | GEO

Gene expression from human iPSC derived cortical neurons

Project description:Gene expression from icas9 human iPSC derived cortical neurons treated with and without doxycycline

| EGAS00001004782 | EGA

Modelling pain states with hiPSC derived sensory neurons

Project description:We report the development of a sensory neuron differentiation protocol which can be used to produce populations of nociceptive sensory neurons. Following transcriptomic and electrophysiological characterisation, we used cultures to investigate molecular pain pathways by exposing cells to three pain related stimuli: heat, cold and a migraine model. Samples were taken at four time points, up to 18 hours after stimulus exposure. Bulk RNA sequencing of samples showed that for temperature models, gene expression changes were dominated by those involved in cell stress and cell death mechanisms. For the migraine model, we obseved a complex pattern of exposure-associated gene expression changes, including the up-regulation of several genes and pathways involved in neuronal and synaptic function.

2022-11-17 | GSE187345 | GEO

Transcriptional effects of the myeloid cell-derived growth factor Oncomodulin on human monocytes and ipSC-derived sensory neurons

Project description:To evaluate the possible translational relevance of oncomodulin (Ocm) and its receptor, we investigated the effect of Ocm on human peripheral blood monocytes and human induced pluripotent stem cells (iPSCs) derived sensory neurons

2023-06-01 | GSE221489 | GEO

RNA sequencing determines nuclear-accumulated Cav3.2iPA on transcriptional regulation of human iPSC-derived sensory neurons

Project description:RNA sequencing determines nuclear-accumulated Cav3.2iPA on transcriptional regulation of human iPSC-derived sensory neurons

| PRJNA1106834 | ENA