Project description:Non coding RNA (ncRNA), such as miRNA, lncRNA and circRNA, has been found to play an important role in the mechanism of plant defense against pathogens. Lychee downy blingt is the main disease of litchi fruit, inflorescence and leaf, which seriously affects the yield of lychee. In this study, we carried out a P.litchii infection experiment on lychee fruits and leaves, and the mRNA and ncRNA in the treated fruits and leaves were analyzed by whole-transcriptome RNA sequencing technology. our work will Provide new insights for the resistance of lychee downy blight .
Project description:To improve the gene annotation and address a series of biological questions, we generated 490,502,822 clean reads of RNA-Seq data from nine tissue types of 'sijimi' longan, including root, stem, leaf, flower_bud, flower, young fruit, pericarp, pulp, and seed, and used them for mapping, and annotation of the longan genome sequence. About 53.55 ~79.40 % of the unique RNA sequences from nine RNA-seq data could be mapped to the genome. RNA-Seq data confirmed a majority of annotated introns, identified thousands of novel alternatively spliced mRNA isoforms, extend gene, SNP and indel, indicative of more functional variation than represented by the gene set alone, and a collection of potentially new and longan-specific gene. A comparative analysis of differential expression in the gene family at the nine different developmental stages showed that most of significant differentially expressed genes were mainly involved in the metabolic pathway, plant- pathogen interaction, and biosynthesis of secondary metabolities, which was fully consistent with the standpoint of D. longan specise containing a lot of plant pahtogen resistant genes, and in particular containing high levels of polyphenolic compounds
Project description:Lychee (Litchi chinensis Sonn.) is a popular fruit worldwide. Lychee downy blight, caused by Peronophythora litchii (P. litchii), is one of the major diseases in lychee. In this study, we conducted a P. litchii infection experiment on the lychee leaves of GW and SFZ, and analyzed the mRNA in the treated leaves using transcriptome RNA sequencing technology. Our research will provide a preliminary basis for the prevention and control of lychee downy blight.
Project description:Non coding RNA (ncRNA), such as miRNA, lncRNA and circRNA, has been found to play an important role in the mechanism of plant defense against pathogens. Lychee downy blingt is the main disease of litchi fruit, inflorescence and leaf, which seriously affects the yield of lychee. In this study, we carried out a P.litchii infection experiment on lychee fruits and leaves, and the mRNA and ncRNA in the treated fruits and leaves were analyzed by whole-transcriptome RNA sequencing technology. our work will Provide new insights for the resistance of lychee downy blight .
2023-02-06 | GSE222651 | GEO
Project description:RNA-SEQ data for four different varieties of Vitis vinifera over four different periods
Project description:To uncover a suit of genes related to the consumer preferred flavours, whole RNA sequencing followed by de novo genome assembly was performed on extreme flavoured papaya varieties RB1 (preferred with sweet flavour and floral aroma) and 1B (non-preferred with bitter flavour and musty aroma) fruits at ripe and unripe stages. We then performed gene expression profiling analysis using data obtained from RNA-seq of 2 different papaya varieties at ripe and unripe stages.
2022-09-19 | GSE213436 | GEO
Project description:RNA-seq sequencing data of some soybean varieties
Project description:Pepper(Capsicum annuum L.) fruit development is a complex and genetically programmed process, a comparative study of transcriptome and proteome changes during two varieties of pepper development（IMG, MG, Br and MR） has been carried out by using RNA-Seq and Lable-free quantitation technology.
