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Penicillium janthinellum

ABSTRACT: Penicillium janthinellum Raw sequence reads

PROVIDER: PRJNA779195 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	SRR16940547_1.fastq.gz	Fastqsanger.gz
	SRR16940547_2.fastq.gz	Fastqsanger.gz
	SRR16940548_1.fastq.gz	Fastqsanger.gz
	SRR16940548_2.fastq.gz	Fastqsanger.gz
	SRR16940549_1.fastq.gz	Fastqsanger.gz

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Impact of maturation and growth temperature on transcription profiles of Penicillium roqueforti conidia

Project description:Purpose: The goal of this study is to compare differences between Penicillium roqueforti conidia from various conditions shown to affect heat-resistance to identify genes possibly related to heat-resistance using RNA-seq. Methods: mRNA from Penicillium roqueforti conidia produced at different temperatures (15C, 25C or 30C) and different cultivation times (3, 5, 7 or 10 days) was extracted, in triplicate, and sequenced with Illumina NextSeq500. The sequence reads that passed quality filters were analyzed using Hisat2 followed by Cufflinks. Results: we mapped about 19 million sequence reads per sample to the Penicillium roqueforti genome (build LCP96 04111) and identified 9723 transcripts in the conidia. Data analysis revealed a subset of 33 genes showing increased expression in culture conditions that increase heat-resistance. Conclusions: Our study generated insight into multiple genes that are likely involved in the heat-resistance of Penicillium roqueforti.

2020-04-24 | GSE149235 | GEO

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