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Phaeodactylum tricornutum strain:UTEX 646

ABSTRACT: Effect of light intensity on Fucoxanthin accumulation in Phaeodactylum tricornutum

PROVIDER: PRJNA779221 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR16915482_1.fastq.gz	Fastqsanger.gz
	SRR16915482_2.fastq.gz	Fastqsanger.gz
	SRR16915483_1.fastq.gz	Fastqsanger.gz
	SRR16915483_2.fastq.gz	Fastqsanger.gz
	SRR16915484_1.fastq.gz	Fastqsanger.gz

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Phaeodactylum tricornutum strain:UTEX 646

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Project description:We have done next generation sequencing of optically thin, exponentialy growing Phaeodactylum tricornutum cultures grown with and without nitrogen source to improve our undestanding of the pathways regulation under conditions that promote lipid accumulation (N-starvation). 3 biologically independent exponentially growing culture of Phaeodactylum tricornutum were pelleted and washed several times with N-free media. Each culture was devided to 2 replicates with initial cell concentration of 2X105 cells/mL, one with NaNO3 as nitrogen source and the other without any nitrogen source. The cuture were bubbled foro 48 hours and sampled for transcriptome together with other physiological parameters and lipid analysis.

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Molecular responses to prolonged darkness and subsequent acclimation to re-illumination in the diatom Phaeodactylum tricornutum

Project description:We have investigated both the response to prolonged darkness and the re-acclimation to “moderate intensity” white irradiance (E = 100 µmol m-2 s-1) in the diatom Phaeodactylum tricornutum, using an integrated approach involving global transcriptional profiling, pigment analyses, imaging and photo-physiological measurements. The responses were studied during continuous white light, after 48 h of dark treatment and after 0.5 h, 6 h, and 24 h of re-exposure to the initial irradiance.

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