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Next Generation Sequencing Facilitates Quantitative Analysis of LPS treated THP-1 cells' Transcriptomes

ABSTRACT: Next Generation Sequencing Facilitates Quantitative Analysis of LPS treated THP-1 cells' Transcriptomes

PROVIDER: PRJNA779446 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR16912752_1.fastq.gz	Fastqsanger.gz
	SRR16912752_2.fastq.gz	Fastqsanger.gz
	SRR16912753_1.fastq.gz	Fastqsanger.gz
	SRR16912753_2.fastq.gz	Fastqsanger.gz
	SRR16912754_1.fastq.gz	Fastqsanger.gz

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Next Generation Sequencing Facilitates Quantitative Analysis of LPS treated THP-1 cells' Transcriptomes

Project description:In order to understand the consequences of the LPS from different bacterias, we analyzed RNA gene expression profiles in a human myeloid cell line THP-1 cells. RNA-seq transcriptome was analyzed in THP-1 cells treated with LPS from three bacteria B. fungorum, S. marcescens, and P. myrsinacearum.

2021-11-11 | GSE188578 | GEO

Next Generation Sequencing Facilitates Quantitative Analysis of dendritic cell Transcriptomes during LPS response

Project description:Purpose:The purpose of this study is to detect activated or silenced genes during LPS-induced dendritic cell. Gene expression differences between two samples could be found using transcriptome profiling (RNA-seq) analysis. Methods:Mouse dendritic cells were generated from bone marrow cells in RPMI-1640 medium with recombinant mouse GM-CSF and IL-4, immature DCs were obtained before or after LPS stimulation. Immature DCs were sorted respectively based on marker CD86 and Iab(MHCII) using flowcytrometer. DC mRNA profiles were generated by deep sequencing,using Illumina. Results: We mapped about 10 million sequence reads per sample to the mouse genome, identified hundreds of genes with significant mRNA variation during LPS stimulation. DC mRNA profiles immature BMDCs were generated by deep sequencing

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Project description:Next Generation Sequencing Facilitates Quantitative Analysis of THP-1 cells under different stimulas.

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