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ABSTRACT: tobacco target spot azoxystrobin 16S sequence

PROVIDER: PRJNA779579 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR16915520.fastq.gz	Fastqsanger.gz
	SRR16915521.fastq.gz	Fastqsanger.gz
	SRR16915522.fastq.gz	Fastqsanger.gz
	SRR16915523.fastq.gz	Fastqsanger.gz
	SRR16915524.fastq.gz	Fastqsanger.gz

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plant metagenome

Project description:Smokeless Tobacco Bacterial Communities raw 16S sequence reads

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Project description:MicroRNAs (miRNAs) are ~21nt long endogenous non-coding RNAs that are involved in post-transcriptional silencing of target mRNAs. miRNAs are processed from precursors having a stem-loop structure that are recognized and cleaved by Dicer-like 1 (DCL1) with the help of dsRNA binding protein Hyponastic leaves 1 (HYL1) and a zinc finger protein named Serrate (SE). Plant miRNA stem-loops are very diverse in length and structure than animal miRNAs. Hence, mechanism of miRNA biogenesis from their precursors is poorly understood. Our bioinformatic analysis predicted that plant miRNAs have unique sequence signature. An artificial precursor with different predicted sequence signatures was constructed. This construct was expressed transiently in tobacco leaves and small RNAs were sequenced. Our analysis revealed that indeed the predicted sequence signature is prefered by plant miRNA biogenesis machinery.

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