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RNA-Seq analysis in human trophoblast stem cells (CT27) upon depletion of Hippo signaling Component TAZ (WWTR1)

ABSTRACT: RNA-Seq analysis in human trophoblast stem cells (CT27) upon depletion of Hippo signaling Component TAZ (WWTR1)

PROVIDER: PRJNA780018 | ENA |

REPOSITORIES: ENA

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RNA-Seq analysis in human trophoblast stem cells (CT27) upon depletion of Hippo signaling Component TAZ (WWTR1)

Project description:The hippo signaling cofactor TAZ (Also known as WWTR1) regulates expression of genes upon interaction with TEAD transcription factors. Among the TEAD transcription factors, TEAD4 is implicated in regulation of the self-renewal process of human trophoblast stem cells. However, we have a poor understanding about the contribution of TAZ, a co-factor of TEAD4, in that process. Thus, the goal of this study is to define the importance of TAZ in orchestrating global gene expression program in human trophoblast stem cells. Using a RNA interference strategy (RNAi), we depleted TAZ expression in human trophoblast stem cells and compared global gene expression patterns via RNA sequencing (RNA-seq). Three individual experiments were performed. The submitted files contain the data (raw fastq files, count data and deseq2 output) from those experiments.

2022-08-08 | GSE188738 | GEO

Human Cardiac Fibroblast BioID2 Interactome for SKI and TAZ(WWTR1)

Project description:We used adenoviral-mediated overexpression of MYC-BioID2, MYC-BioID2-SKI, MYC-BioID2-WWTR1 (TAZ) in human primary cardiac fibroblasts to elucidate the interaction between SKI and the Hippo signaling pathway. Original data is also available on the Global Proteome Machine (http://hs2.proteome.ca/tandem/thegpm_tandem.html). Datasets are identified as follows: GPM10000002938 and 2939 are untreated negative control cell lysates; GPM10000002941 and 2942 are "empty" MYC-BioID2 vector; GPM10000002943 and 2944 are MYC-BioID2-SKI; and GPM10000002944 and 2945 are MYC-BioID2-WWTR1(TAZ).

2021-09-09 | PXD018246 | Pride

Expression profile of RCC4 cells with TAZ (WWTR1) silencing

Project description:TAZ, also known as WWTR1, is the one of the effectors of Hippo pathway. With its paralog, YAP, TAZ promotes organ size growth as well as tumor metastasis. In human renal carcinoma cells, we found that TAZ-silencing induces resisntance toward erastin-induced ferroptosis. In this study, TAZ is silencing in clear cell carcinoma cell line RCC4 to elucidate the downstream targets that promotes the resistance toward erastin-induced ferroptosis.

2019-09-03 | GSE121689 | GEO

Deciphering the involvement of the Hippo pathway co-regulators, YAP/TAZ in invadopodia formation and matrix degradation

Project description:The paralogous transcriptional cofactors Yes-associated protein (YAP) and transcriptional co-activator with PDZ-binding motif (TAZ, also called WWTR1), the main effectors of the Hippo signal transduction pathway, are emerging as pivotal determinants of malignancy in cancer. We aim to compare the transcriptome altered by knockdown of YAP, TAZ or both using siRNA in MDA-MB-231 (derived from human metastatic breast adenocarcinoma).

2023-03-20 | GSE205726 | GEO

Regulation of gene expression by the Hippo effectors, Taz and Yap, in muscle stem cells

Project description:The Hippo pathway downstream effectors, Yap and Taz, play key roles in cell proliferation and tissue growth, regulating gene expression especially via interaction with Tead transcription factors. To investigate their role in skeletal muscle stem cells, we analysed gene expression changes driven by Taz and compared these to Yap mediated changes to the transcriptome by measurement of gene expression on Affymetrix microarrays. To interrogate overlapping and unique transcriptional changes driven by these Hippo effectors, satellite cell-derived myoblasts were transduced with constitutively active TAZ S89A or YAP S127A retrovirus for 24h or 48h, with empty retrovirus as control. Triplicate microarray analyses of empty vector controls, hYAP1 S127A and TAZ S89A transgenic primary myoblasts were conducted.

2017-06-20 | E-MTAB-5395 | biostudies-arrayexpress

Muscle stems cell LC-MS - Common and Distinctive Functions of the Hippo Effectors Taz and Yap in Skeletal Muscle Stem Cell Function

Project description:Abstract Hippo pathway downstream effectors Yap and Taz play key roles in cell proliferation and regeneration, regulating gene expression especially via interaction with Tead transcription factors. To investigate their role in skeletal muscle stem cells, we analysed Taz in vivo and ex vivo in comparison to Yap. Taz was expressed in activated satellite cells. siRNA knockdown or constitutive expression of wildtype or constitutively active TAZ mutants showed that TAZ promoted proliferation, a function that was shared with YAP. However, at later stages of myogenesis, TAZ also enhanced myogenic differentiation of myoblasts, whereas YAP inhibits such differentiation. Functionally, while muscle growth was mildly affected in Taz (gene symbol Wwtr1-/-) knockout mice, there were no overt effect on regeneration. However, conditional knockout of Yap in satellite cells of Pax7Cre-ERT2/+ : Yapflox/flox : Rosa26Lacz mice produced a marked regeneration deficit. To identify potential mechanisms, microarray analysis showed many common Taz/Yap targets, but Taz also regulates some genes independently of Yap, including myogenic genes such as Pax7, Myf5 and Myod1. Proteomic analysis of Yap/Taz revealed many common binding partners, but Taz also interacts with proteins distinct from Yap, that are mainly involved in myogenesis and aspects of cytoskeleton organization. Neither TAZ nor YAP bind members of the Wnt destruction complex but both extensively changed expression of Wnt and Wnt-cross talking genes with known roles in myogenesis. Finally, TAZ operates through Tead4 to enhance myogenic differentiation. In summary, Taz and Yap have overlapping functions in promoting myoblast proliferation but Taz then switches to promote myogenic differentiation.

2017-06-13 | PXD005751 | Pride

DUB1 suppresses Hippo signaling via modulating TAZ protein in gastric cancer

Project description:The Hippo pathway functions as a tumor-suppressor pathway in human cancers, while the dys-function of Hippo pathway is frequently observed in malignancies. Although the YAP/TAZ activity is tightly controlled by the phosphorylation cascade of MST-LATS-YAP/TAZ axis, it is still unclear why YAP/TAZ protein is activated in human cancers, even Hippo pathway is still active. Besides phosphorylation, recent studies implicate that several post-translational modifications also play critical roles in modulating TAZ function, including ubiquitination. Here, by a DUB (Deubiquitinases) siRNA screening library, we discovered DUB1 as a critical modulator to facilitate gastric cancer stemness and progression, which deubiquitinated and activated TAZ protein. We also identified DUB1 was elevated in gastric cancer, which correlated with TAZ activation and poor survival. DUB1 associated with TAZ protein and deubiquitinated TAZ at several lysine sites, which subsequently stabilized and facilitated TAZ function. Our study revealed a novel deubiquitinase of Hippo/TAZ axis and one possible therapeutic target for Hippo-driven gastric cancer.

2022-02-12 | GSE196262 | GEO

The lncRNA TAZ-AS202 promotes lung cancer progression through stabilization of the E2F1 transcription factor and activation of Ephrin signaling

Project description:Silencing of TAZ (WWTR1) and lncRNA TAZ-AS202 (WWTR1-AS1-202) by siRNA transfection on A549 cell line of lung cancer

2023-12-07 | E-MTAB-12507 | biostudies-arrayexpress

Functional characterizations of the Hippo pathway effectors YAP and TAZ

Project description:The Hippo pathway plays an important role in regulating tissue homeostasis, and its effectors YAP and TAZ are responsible for mediating the vast majority of its physiological functions. Although YAP and TAZ are thought to be largely redundant and similarly regulated by Hippo signaling, they have developmental, structural, and physiological differences which suggest there may be differences in their regulation and downstream functions. To better understand the functions of YAP and TAZ in the Hippo pathway, we generated knockout cells and evaluated them in response to many conditions and stimuli. Here, we used RNA-seq to identify and compare differences in the transcriptional profiles between the YAP and TAZ.

2018-05-25 | GSE111583 | GEO

Loss-of-Function of the Hippo Transducer TAZ Reduces Mammary Tumor Growth through a Myeloid-Derived Suppressor Cell-Dependent Mechanism

Project description:TAZ, a key component in Hippo pathway, plays an important role in breast tumorigenesis. To understand the entrisic as well as extrinsic role of TAZ in mammary tumors, we performed RNA-seq analysis using mammary tumors generated from sgControl or sgTaz 4T1 cells.

2023-05-31 | GSE205160 | GEO

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