Similar Datasets
Project description:De novo assembly of immature gonadal transcriptome was performed to identify genes involved in gonadal development. A total of 81,757 and 43,257 transcripts were obtained from the immature testicular and ovarian transcriptomes, respectively. About 84,367 unigenes were constructed after removing redundancy which were a representation of both the gonadal transcriptomes. About 298 differentially expressed genes were identified. The present study identified certain important genes/factors involved in the gonadal development of C. carpio which may provide insights into the understanding of sex differentiation and gonadal development processes.
2019-08-19 | GSE112157 | GEO
Project description:Sablefish gonadal transcriptome sequencing
| PRJNA416142 | ENA
Project description:Japanese flounder gonadal transcriptome
| PRJNA321665 | ENA
Project description:The unraveling of heterogenous cell populations, — previously masked in bulk transcriptome measurements — are made possible through analysis of single-cell transcriptomics. The goals of this study are to examine single-cell NGS-derived gonadal cell transcriptome profiling and to evaluate heterogeneity of gonadal germ cells in avian species. We performed single-cell RNA sequencing using embryonic gonadal cells of HH stage 28 from male and female zebra finches. We identified 12,489 male transcripts and 10,046 female transcripts. We annotated 9 different cell types from a total of 20 clustered single cells and identified that primordial germ cells, which have been considered as single population, can be distinguishable into three subtypes. The three PGC subtypes were specifically enriched for genes with expression patterns related to germness or pluripotency, suggesting functional differences in PGCs according to the three subtypes. Our study represents the first single-cell RNA seq analysis of gonadal transcriptomes in zebra finch. Our results revealed the first heterogeneity and intrinsic changes of gonadal primordial germ cells, which have hitherto been considered as a single population in avian species. We conclude that single-cell RNA seq based transcriptome analysis would offers new perspectives on avian primordial germ cells.
2021-12-13 | GSE177478 | GEO
Project description:This repository contains whole genome long read sequencing data generated using Oxford Nanopore Technologies from a mouse of the Four Core Genotypes cross. By crossing mice with both a deletion of the sex-determining factor Sry on the Y-chromosome and a transgenic insertion of Sry on Chromosome 3, four combinations of gonadal (testis or ovaries) and chromosomal (XX or XY) are generated, namely XYSry-Chr3Sry+ (gonadal and chromosomal males), XYSry- (gonadal females, chromosomal males), XX (gonadal and chromosomal females), XXChr3Sry+ (gonadal males, chromosomal females). The transgenes were on a C57BL6 genetic background which is crossed with a CAST/EiJ female to allow for the distinction of the parental haplotypes. DNA sequencing was done on a liver sample of the XYSry+ genotype.
2024-10-14 | E-MTAB-14291 | biostudies-arrayexpress
Project description:Gallus gallus gonadal tissue transcriptome
| PRJNA1142492 | ENA
Project description:Mouse fetal gonadal transcriptome analysis
| PRJNA320667 | ENA