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ABSTRACT: Effect of Dendrobium officinale on the transcriptome of the liver of Mus musculus albus

PROVIDER: PRJNA780764 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR16956400_1.fastq.gz	Fastqsanger.gz
	SRR16956400_2.fastq.gz	Fastqsanger.gz
	SRR16956401.fastq.gz	Fastqsanger.gz
	SRR16956401_1.fastq.gz	Fastqsanger.gz
	SRR16956401_2.fastq.gz	Fastqsanger.gz

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Project description:Dendrobium officinale is a traditional medicinal herb with a mount of bioactive components. Alkaloid is one of the major active ingredients of Dendrobium plants, and its immune regulatory effects have been well-studied. A total of 4857 DEGs, including 2943 up- and 1932 down-regulated genes, were identified between the control and MeJA-treated groups. Several shikimate and methylerythritol 4-phosphate pathway genes and a number of MeJA-induced P450 family genes, aminotransferase genes and methyltransferase genes were identified, providing several important candidates to further elucidate the alkaloid biosynthetic pathway of D. officinale. Furthermore, a large number of MeJA-induced transcript factor encoding genes were identified, suggesting a complex genetic network affecting the alkaloid metabolism in D. officinale.

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Project description:We explored the microevolutionary trends of CTCF binding evolution by preforming ChIP-seq experiments in five closely related Mus strains, subspecies and species: Mus musculus domesticus, Mus musculus castaneus, Mus spretus, Mus caroli and Mus pahari. All experiments were performed in adult male liver samples in 3 biological replicates and with an input control set. Complementary RNA-seq data from this same study have been deposited in ArrayExpress under accession numebr E-MTAB-5768 ( https://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-5768 ).

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