Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR16988200_1.fastq.gz | Fastqsanger.gz | |||
| SRR16988200_2.fastq.gz | Fastqsanger.gz | |||
| SRR16988201_1.fastq.gz | Fastqsanger.gz | |||
| SRR16988201_2.fastq.gz | Fastqsanger.gz | |||
| SRR16988202_1.fastq.gz | Fastqsanger.gz |
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Project description:Bermudagrass (Cynodon dactylon L.) is an important warm-season turfgrass species with well-developed stolons, which lay the foundation for fast propagation of bermudagrass plants through asexually clonal growth. However, the growth and development of bermudagrass stolons are still poorly understood at the molecular level. In this study, we comprehensively analyzed the succinylation modifications of proteins in fast growing stolons of bermudagrass cultivar Yangjiang. A total of 226 lysine succinylation sites on 128 proteins were successfully identified using liquid chromatography coupled to tandem mass spectrometry.
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Project description:Bermudagrass (Cynodon dactylon L.) is an important warm-season turfgrass species with well-developed stolons, which lay the foundation for fast propagation of bermudagrass plants through asexually clonal growth. However, the growth and development of bermudagrass stolons are still poorly understood at the molecular level. In this study, we comprehensively analyzed the acetylation modifications of proteins in fast growing stolons of bermudagrass cultivar Yangjiang. A total of 4657 lysine acetylation sites on 1914 proteins were successfully identified using liquid chromatography coupled to tandem mass spectrometry.
2022-08-20 | PXD034841 | Pride
Project description:Bermudagrass is an important warm-season turfgrass species with both erect growing shoots and prostrate growing stolons, however, the mechanism how bermudagrass shoots and stolons form and maintain their unique geotropic growth modes are still unclear. In this study, we compared the proteome of the internode section of shoots and stolons at the same developmental stage in bermudagrass cultivar Yangjiang. The results indicated that 376 protein species were differentially accumulated in the two types of stems.
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Project description:As a widely used turfgrass species, bermudagrass (Cynodon dactylon L.) can be easily propagated by colonial growth of stolons. Previous studies collectively revealed that exotic environmental factors and intrinsic hormones and genes are all involved in the differentiation, development, and diageotropically growth of stolons. However, the detailed molecular mechanism how environmental and hormone signals regulate the gene expression and biochemical activities in bermudagrass stolons remains unclear. In this study, LC-MS/MS analyses of the total protein extracts of bermudagrass stolons without preliminary phosphopeptide-enrichment successfully identified 862 nonredundant phosphorylation sites and 613 phosphoproteins.
2019-09-25 | PXD014113 | Pride
Project description:The clinical importance of microbiomes to the chronicity of wounds is widely appreciated, yet little is understood about patient-specific processes shaping wound microbiome composition. Here, a two-cohort microbiome-genome wide association study is presented through which patient genomic loci associated with chronic wound microbiome diversity were identified. Further investigation revealed that alternative TLN2 and ZNF521 genotypes explained significant inter-patient variation in relative abundance of two key pathogens, Pseudomonas aeruginosa and Staphylococcus epidermidis. Wound diversity was lowest in Pseudomonas aeruginosa infected wounds, and decreasing wound diversity had a significant negative linear relationship with healing rate. In addition to microbiome characteristics, age, diabetic status, and genetic ancestry all significantly influenced healing. Using structural equation modeling to identify common variance among SNPs, six loci were sufficient to explain 53% of variation in wound microbiome diversity, which was a 10% increase over traditional multiple regression. Focusing on TLN2, genotype at rs8031916 explained expression differences of alternative transcripts that differ in inclusion of important focal adhesion binding domains. Such differences are hypothesized to relate to wound microbiomes and healing through effects on bacterial exploitation of focal adhesions and/or cellular migration. Related, other associated loci were functionally enriched, often with roles in cytoskeletal dynamics. This study, being the first to identify patient genetic determinants for wound microbiomes and healing, implicates genetic variation determining cellular adhesion phenotypes as important drivers of infection type. The identification of predictive biomarkers for chronic wound microbiomes may serve as risk factors and guide treatment by informing patient-specific tendencies of infection.
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