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Early-life thermal stress mediates long-term alterations in hypothalamic microglia [RRBS]

ABSTRACT: Early-life thermal stress mediates long-term alterations in hypothalamic microglia [RRBS]

PROVIDER: PRJNA783616 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR17048758.fastq.gz	Fastqsanger.gz
	SRR17048759.fastq.gz	Fastqsanger.gz
	SRR17048760.fastq.gz	Fastqsanger.gz
	SRR17048761.fastq.gz	Fastqsanger.gz
	SRR17048762.fastq.gz	Fastqsanger.gz

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Early-life thermal stress mediates long-term alterations in hypothalamic microglia [RRBS]

Project description:We identified the microglial population in the chick anterior hypothalamus (AH) and showed that early-life thermal stress (on day 3 post-hatch) influences the inflammatory process in the AH. We described alterations in genome-wide CpG methylation profile of hypothalamic microglia between heat-conditioned and non-conditioned 10-day-old chicks prior LPS challenge. We suppose that changes in CpG DNA methylation might be involved in reprogramming microglial function.

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Early-life thermal stress mediates long-term alterations in hypothalamic microglia

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Early-life thermal stress mediates long-term alterations in hypothalamic microglia [RNA-Seq]

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Early-life thermal stress mediates long-term alterations in hypothalamic microglia [RNA-Seq]

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Early-life thermal stress mediates long-term alterations in hypothalamic microglia

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Long Term Thermal Injury Analysis

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Project description:To better understand the epigenetic mechanism underlying pubertal onset, the hypothalamic genome-wide chromatin accessibility patterns in mouse arcuate nucleus at early and late pubertal stages were explored. Female mice have been widely used in multiple studies on pubertal development as they present the similar molecular behaviors in HPG axis and stable cycles of menstrual calendar like human. Hypothalamic ARC underwent a huge epigenetic and genetic reprogramming to adapt to the response and feedback on sexual hormones during the stages of early pubertal (2-5-week of age) and late puberty (5-8-week of age) . We harvested 4- and 8-week hypothalamic ARC and employed ATAC-seq on a genome-wide scale. Combined with previous RRBS, RRHP and RNA-seq, the connections between DNA (hydroxyl)methylation in retroelements and gene expression were studied, emphasizing the importance of epigenetic alterations in regulating transcription in puberty onset.

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Thermal transcriptome

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