Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Single cell RNAseq of murine gliomas induced by PDGFB overexpression

ABSTRACT: Single cell RNAseq of murine gliomas induced by PDGFB overexpression

PROVIDER: PRJNA783640 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR17038763_1.fastq.gz	Fastqsanger.gz
	SRR17038763_2.fastq.gz	Fastqsanger.gz
	SRR17038764_1.fastq.gz	Fastqsanger.gz
	SRR17038764_2.fastq.gz	Fastqsanger.gz
	SRR17038765_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 16

Similar Datasets

Bulk RNAseq of murine gliomas driven by overexpression of PDGFB

Project description:Bulk RNAseq of murine gliomas driven by overexpression of PDGFB

| PRJNA783316 | ENA

Single cell RNAseq of murine gliomas induced by PDGFB overexpression

Project description:Glioblastoma progression remains elusive, especially in its first stages. Here, by simultaneous transfer of PDGFB and genetic barcode in mouse brain, we triggered gliomagenesis in univocally labelled cells in vivo, hence enabling direct tracing of glioblastoma evolution from the earliest possible stage. We observed an unexpectedly high incidence of clonal extinction events and progressive clonal size divergence, even after the acquisition of a malignant phenotype. Computational modelling suggests these dynamics as the consequence of a clonal-based cell-cell competition. Bulk and single-cell transcriptome analyses, coupled to lineage tracing, revealed that the strongest correlation with clonal size unbalancing is shown by Myc transcriptional targets. Additionally, higher levels of Myc signalling discriminate "winner" from "loser" clones in early gliomagenesis. These results agree with recent understanding of the Myc-mediated induction of supercompetitive phenotype in mammalian embryo. Our findings shed light on aspects of glioblastoma evolution inaccessible by conventional retrospective approaches and highlight the potential of the combined use of clonal tracing and transcriptomic analyses in this field.

2023-07-18 | GSE189577 | GEO

Bulk RNAseq of murine gliomas driven by overexpression of PDGFB

2023-07-18 | GSE189517 | GEO

RNAseq of murine models of high-grade gliomas induced by overexpression of PDGF-B or EGFRvIII

Project description:RNAseq of murine models of high-grade gliomas induced by overexpression of PDGF-B or EGFRvIII

| PRJNA431534 | ENA

Genome-wide maps in PDGFB-induced glioma

Project description:We performed genome-wide profiling of oligodendrocyte lineage transcription factor 2 (Olig2) and other histone markers in platelet-derived growth factor subunit B (PDGFB)-induced glioma and genome-occupancy analyses coupled with transcriptome profiling to reveal gene regulatory network. Examination of Olig2, H327Ac, and H3K4me3 genome-wide occupancy in PDGFB-induced Ctrl-T and Olig2cKO brain tumors (gliomas).

2016-05-17 | E-GEOD-80088 | biostudies-arrayexpress

RNAseq analysis of murine brainstem gliomas with and without H3.3K27M

Project description:Using the RCAS/tv-a system, we induced murine brainstem gliomas (PDGF-B; p53 loss using RCAS-Cre with and without H3.3K27M) in Nestin tv-a; p53 floxed mice

2017-05-11 | GSE98765 | GEO

Expression data from PDGF-B induced murine gliomas at different progression stages

Project description:The different phases of tumor immunoediting in vivo were dissected thanks to a murine model of glioma induced by PDGF-B overexpression. We show that low-grade gliomas are highly immunostimulatory and that the adaptive immune system prevents the development of secondary tumor in syngeneic mice. During tumor progression, glioma cells downregulate immunostimulatory genes and the immune infiltrate becomes pro-tumorigenic.

2018-10-22 | GSE108954 | GEO

Novel cancer gene discovery using a forward genetic screen in RCAS-PDGFB driven gliomas

Project description:Novel cancer gene discovery using a forward genetic screen in RCAS-PDGFB driven gliomas

| PRJNA854593 | ENA

RNAseq of Pdgfb' brain cells obtained by from young male mice

Project description:RNAseq of Pdgfb' brain cells obtained by from young male mice

| PRJNA927327 | ENA

Novel cancer gene discovery using a forward genetic screen in RCAS-PDGFB driven gliomas

Project description:Here we describe the use of a high-throughput pipeline coupled to the commonly used tissue-specific retroviral RCAS-TVA mouse tumor model system. Utilizing next generation sequencing, we show that retroviral integration sites can be reproducibly detected in malignant stem cell lines generated from RCAS-PDGFB-driven glioma biopsies.

2022-07-05 | GSE207264 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data