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Urinary sediment mRNA level of CREBBP and CYBA in children with steroid-resistant nephrotic syndrome

ABSTRACT: Urinary sediment mRNA level of CREBBP and CYBA in children with steroid-resistant nephrotic syndrome

PROVIDER: PRJNA784291 | ENA |

REPOSITORIES: ENA

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Urinary sediment mRNA level of CREBBP and CYBA in children with steroid-resistant nephrotic syndrome

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Label-free mass spectrometry-based profiling of urinary proteins and metabolites for non-invasive diagnosis of idiopathic nephrotic syndrome in children

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Predicting and Defining Steroid Resistance in Pediatric Nephrotic Syndrome using Plasma Metabolomics

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proteomic analysis of the formalin-fixed paraffin embedded (FFPE) renal tissue between steroid-sensitive and steroid-resistant (SR) children FSGS patients

Project description:In children, focal segmental glomerulosclerosis (FSGS) is the main cause of steroid resistant nephroticsyndrome (SRNS). To identify specific candidates and the mechanism of steroid resistance, we examined the formalin-fixed paraffin embedded (FFPE) renal tissue protein profiles via liquid chromatography tandem mass spectrometry (LC-MS/MS). Renal biopsies from seven steroid-sensitive (SS) and eleven steroid-resistant (SR) children FSGS patients were obtained. We examined the formalin-fixed paraffin embedded (FFPE) renal tissue protein profiles via liquid chromatography tandem mass spectrometry (LC-MS/MS). Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment and Gene Ontology (GO) analysis, as well as the construction of protein-protein interaction (PPI) network were performed. Two proteins were further valiadated by immunohistochemistry staining in FSGS patients and mice models. In total, we quantified 4794 proteins, of which 325 were found to be differentially expressed proteins (DEPs) between the SS and SR group (foldchange ≥ 2, P＜0.05). The results of GO revealed that the most significant up-regulated proteins were primarily related to protein transportation, regulation of the complement activation process and cytolysis. Moreover, clustering analysis showed differences in the pathways (lysosome, terminal pathway of complement) between the two groups. Among these potential candidates, validation analyses for LAMP1 and ACSL4 were conducted. LAMP1 was observed to have a higher expression in glomerulus, while ACSL4 was expressed more in tubular epithelial cells.

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Gene expression profiles of peripheral blood mononuclear cells (PBMC) from children with active idiopathic nephrotic syndrome (INS) and healthy controls (HC) [HC_vs_INS]

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